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Titolo:
Bladder stretch alters urinary heparin-binding epidermal growth factor andantiproliferative factor in patients with interstitial cystitis
Autore:
Chai, TC; Zhang, CO; Shoenfelt, JL; Johnson, HW; Warren, JW; Keay, S;
Indirizzi:
Univ Maryland, Div Urol, Sch Med, Dept Surg, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 , Dept Surg, Baltimore, MD 21201 USA Univ Maryland, Sch Med, Div Infect Dis, Dept Internal Med, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 nternal Med, Baltimore, MD 21201 USA Univ Maryland, Sch Med, Dept Obstet & Gynecol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 t & Gynecol, Baltimore, MD 21201 USA Vet Adm Maryland Hlth Care Syst, Baltimore, MD USA Vet Adm Maryland Hlth Care Syst Baltimore MD USA Syst, Baltimore, MD USA
Titolo Testata:
JOURNAL OF UROLOGY
fascicolo: 5, volume: 163, anno: 2000,
pagine: 1440 - 1444
SICI:
0022-5347(200005)163:5<1440:BSAUHE>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL PERMEABILITY BARRIER; SURFACE GLYCOSAMINOGLYCANS; THERAPY; CELLS;
Keywords:
bladder; growth substances; cystitis, interstitial; heparin; epidermal growth factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Chai, TC Univ Maryland, Div Urol, Sch Med, Dept Surg, 22 S Greene St,S8D18, Baltimore, MD 21201 USA Univ Maryland 22 S Greene St,S8D18 Baltimore MD USA 21201 201 USA
Citazione:
T.C. Chai et al., "Bladder stretch alters urinary heparin-binding epidermal growth factor andantiproliferative factor in patients with interstitial cystitis", J UROL, 163(5), 2000, pp. 1440-1444

Abstract

Purpose: The etiology of interstitial cystitis is unknown. Urine from patients with interstitial cystitis has been shown to inhibit urothelial proliferation through a putative antiproliferative factor and to contain decreased levels of heparin-binding epidermal growth factor-like growth factor (HB-EGF) compared to controls. Stretch of detrusor smooth muscle cells is knownto stimulate HB-EGF production. Because bladder hydrodistention sometimes alleviates the symptoms of interstitial cystitis, we determined whether thestretch stimulus of hydrodistention alters antiproliferative factor activity and/or IIB-EGF in interstitial cystitis urine specimens. Materials and Methods: Urine was collected immediately before, and 2 to 4 hours and 2 weeks after hydrodistention from 15 patients with symptoms and cystoscopic findings compatible with interstitial cystitis and 13 controls. Hydrodistention was performed with the subject under general or regional anesthesia and bladders were distended to 80 cm. water 3 times. Urinary HB-EGF was measured by enzyme-linked immunosorbent assay and urinary antiproliferative factor activity was determined by measuring SII-thymidine uptake bynormal, human bladder urothelial cells. Results: Hydrodistention significantly increased urinary HB-EGF in patients with interstitial cystitis toward normal control values (before distention p = 0.003, 2 weeks after distention p = 0.67). Urine antiproliferative factor activity decreased significantly after hydrodistention in patients with interstitial cystitis. However, antiproliferative factor activity in interstitial cystitis and control specimens was still statistically different 2weeks after distention (before distention p = 0.0000004, 2 weeks after distention p = 0.04). Conclusions: Bladder stretch increased HB-EGF and conversely reduced antiproliferative factor activity in urine from patients with interstitial cystitis but not controls up to 2 weeks after distention. These results provide additional evidence for the possible role of antiproliferative factor and decreased HB-EGF in the pathophysiology of interstitial cystitis. To our knowledge this is also the first human study to show that in vivo bladder stretch can alter urinary factors that regulate cell growth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 05:25:23