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Titolo:
Analysis of VMAT2 binding after methamphetamine or MPTP treatment: Disparity between homogenates and vesicle preparations
Autore:
Hogan, KA; Staal, RGW; Sonsalla, PK;
Indirizzi:
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Neurol, Piscataway, NJ 08854 USA Univ Med & Dent New Jersey Piscataway NJ USA 08854 scataway, NJ 08854 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 5, volume: 74, anno: 2000,
pagine: 2217 - 2220
SICI:
0022-3042(200005)74:5<2217:AOVBAM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON-EMISSION-TOMOGRAPHY; DOPAMINERGIC-NEURONS; C-11 WIN-35,428; NEUROTOXICITY; MOUSE; TRANSPORTERS;
Keywords:
dopamine nerve terminals; methamphetamine; MPTP; vesicular monoamine transporter; striatal homogenates; dihydrotetrabenazine binding;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Sonsalla, PK Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, DeptNeurol, 675 Hoes Lane, Piscataway, NJ 08854 USA Univ Med & Dent New Jersey675 Hoes Lane Piscataway NJ USA 08854
Citazione:
K.A. Hogan et al., "Analysis of VMAT2 binding after methamphetamine or MPTP treatment: Disparity between homogenates and vesicle preparations", J NEUROCHEM, 74(5), 2000, pp. 2217-2220

Abstract

[H-3]Dihydrotetrabenazine ([H-3]DTBZ), a specific ligand for the vesicularmonoamine transporter (VMAT2), has been used to characterize the integrityof monoaminergic nerve terminals in experimental animals and humans. The purpose of the present studies was to compare the loss of VMAT2 binding withthe loss of other neurochemical markers of the dopamine (DA) nerve terminals in mice treated with neurotoxic doses of methamphetamine (METH) or MPTP. Profound decreases (greater than or equal to 70%) in DA content, tyrosine hydroxylase activity, and [H-3]carbomethoxy-3-(4-fluorophenyl)tropane binding to the DA transporter were observed in striatal homogenates at both 1 and 6 days after exposure to the neurotoxins. It is surprising that no significant loss of [H-3]DTBZ binding in the homogenates was observed at 1 day after exposure, although a significant loss (-50%) was apparent 6 days later. However, in isolated vesicle preparations, [H-3]DTBZ binding and active [H-3]DA uptake were markedly reduced (>70%) at 1 day. These observations indicate that vesicle function is compromised at an early time point after exposure to neurotoxic insult. Furthermore, the changes in [H-3]DTBZ binding inhomogenates may not be a sensitive indicator of early damage to synaptic vesicles, although homogenate binding reliably identifies a loss of VMAT2 atlater times.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:01:06