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Titolo:
Regulation of tryptophan hydroxylase activity in Xenopus laevis photoreceptor cells by cyclic AMP
Autore:
Valenciano, AI; Alonso-Gomez, AL; Iuvone, PM;
Indirizzi:
Emory Univ, Dept Pharmacol, Sch Med, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 Pharmacol, Sch Med, Atlanta, GA 30322 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 5, volume: 74, anno: 2000,
pagine: 1961 - 1967
SICI:
0022-3042(200005)74:5<1961:ROTHAI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEROTONIN N-ACETYLTRANSFERASE; CHICK PINEAL CELLS; DEPENDENT PROTEIN-KINASE; CIRCADIAN CLOCK; RETINAL PHOTORECEPTORS; MELATONIN PRODUCTION; VERTEBRATE RETINA; LIGHT; DARK; PHOSPHORYLATION;
Keywords:
retina; tryptophan hydroxylase; melatonin; cyclic AMP; photoreceptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Iuvone, PM Emory Univ, Dept Pharmacol, Sch Med, 1510 Clifton Rd, Atlanta, GA 30322 USA Emory Univ 1510 Clifton Rd Atlanta GA USA 30322 a, GA 30322 USA
Citazione:
A.I. Valenciano et al., "Regulation of tryptophan hydroxylase activity in Xenopus laevis photoreceptor cells by cyclic AMP", J NEUROCHEM, 74(5), 2000, pp. 1961-1967

Abstract

The aim of this study was to investigate the role of cyclic AMP in the regulation of tryptophan hydroxylase activity localized in retinal photoreceptor cells of Xenopus laevis, where the enzyme plays a key role in circadian melatonin biosynthesis. In photoreceptor-enriched retinas that lack serotonergic neurons, tryptophan hydroxylase activity is markedly stimulated by treatments that increase intracellular levels of cyclic AMP or activate cyclic AMP-dependent protein kinase, including forskolin, phosphodiesterase inhibitors, and cyclic AMP analogues. In contrast, cyclic AMP has no effect on tryptophan hydroxylase mRNA abundance. Experiments using cycloheximide and actinomycin D demonstrate that cyclic AMP exerts its regulatory effect via posttranslational mechanisms mediated by cyclic AMP-dependent protein kinase. The effect of cyclic AMP is independent of the phase of the photoperiod,suggesting that the nucleotide is not a mediator of the circadian rhythm of tryptophan hydroxylase. Cyclic AMP accumulation is higher in darkness than in light, as is tryptophan hydroxylase activity. Furthermore, the stimulatory effect of forskolin and that of darkness are inhibited by H89, an inhibitor of cyclic AMP-dependent protein kinase. In conclusion, cyclic AMP maymediate the acute effects of light and darkness on tryptophan hydroxylase activity of retinal photoreceptor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:04:40