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Titolo:
Glutamate-stimulated activation of DNA synthesis via mitogen-activated protein kinase in primary astrocytes: Involvement of protein kinase C and related adhesion focal tyrosine kinase
Autore:
Schinkmann, KA; Kim, TA; Avraham, S;
Indirizzi:
Harvard Univ, Sch Med, Inst Med, BIDMC,Div Expt Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 BIDMC,Div Expt Med, Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA Harvard Univ BostonMA USA 02115 Sch Med, Dept Med, Boston, MA 02115 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 5, volume: 74, anno: 2000,
pagine: 1931 - 1940
SICI:
0022-3042(200005)74:5<1931:GAODSV>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; CULTURED ASTROCYTES; COUPLED RECEPTORS; INDUCED APOPTOSIS; GLIAL-CELLS; CALCIUM; PHOSPHORYLATION; PYK2; MEGAKARYOCYTES; BRAIN;
Keywords:
glutamate; primary astrocytes; mitogen-activated protein kinase; tyrosine kinase; related adhesion focal tyrosine kinase (RAFTK);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Avraham, S Harvard Univ, Sch Med, Inst Med, BIDMC,Div Expt Med, 4 BlackfanCircle,Rm 323, Boston, MA 02115 USA Harvard Univ 4 Blackfan Circle,Rm 323 Boston MA USA 02115 5 USA
Citazione:
K.A. Schinkmann et al., "Glutamate-stimulated activation of DNA synthesis via mitogen-activated protein kinase in primary astrocytes: Involvement of protein kinase C and related adhesion focal tyrosine kinase", J NEUROCHEM, 74(5), 2000, pp. 1931-1940

Abstract

Glutamate is the major excitatory neurotransmitter in the CNS. Although its role in neurons has been studied extensively, little is known about its function in astrocytes. We studied the effects of glutamate on signaling pathways in primary astrocytes. We found that the tyrosine kinase related adhesion focal tyrosine kinase (RAFTK) is tyrosine phosphorylated in response to glutamate in a time- and dose-dependent manner. This phosphorylation was pertussis toxin (PTX) sensitive and could be attenuated by the depletion ofCa2+ from intracellular stores. RAFTK tyrosine phosphorylation was mediated primarily by class I/II metabotropic glutamate receptors and depends on protein kinase C (PKC) activation. Glutamate treatment of primary astrocytesalso results in a significant increase in the activity of the mitogen-activated protein kinases [extracellular signal-related kinases 1/2 (ERK1/2)]. Like RAFTK phosphorylation, ERK1/2 activation is PTX sensitive and can be attenuated by the depletion of intracellular Ca2+ and by PKC inhibition, suggesting that RAFTK might mediate the glutamate-dependent activation of ERK1/2. Furthermore, we demonstrated that glutamate stimulation of primary astrocytes leads to a significant increase in DNA synthesis. Glutamate-stimulated DNA synthesis is PTX sensitive and can be inhibited by the MAP kinase kinase inhibitor PD98059, suggesting that in primary astrocytes, glutamate might signal via RAFTK and MAP kinase to promote DNA synthesis and cell proliferation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 19:21:07