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Titolo:
Development and characterisation of a glutamate-sensitive motor neurone cell line
Autore:
Eggett, CJ; Crosier, S; Manning, P; Cookson, MR; Menzies, FM; McNeil, CJ; Shaw, PJ;
Indirizzi:
Univ Newcastle Upon Tyne, Sch Med, Dept Neurol, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England Univ Newcastle Upon Tyne Newcastle Upon Tyne Tyne & Wear England NE2 4HH Univ Newcastle Upon Tyne, Sch Med, Dept Clin Biochem, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England Univ Newcastle Upon Tyne Newcastle Upon TyneTyne & Wear England NE2 4HH
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 5, volume: 74, anno: 2000,
pagine: 1895 - 1902
SICI:
0022-3042(200005)74:5<1895:DACOAG>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBELLAR GRANULE CELLS; NITRIC-OXIDE; RADICAL PRODUCTION; P19 CELLS; NEUROTOXICITY; DEATH; EXCITOTOXICITY; PEROXYNITRITE; PATHOGENESIS; SUPEROXIDE;
Keywords:
excitotoxicity; NSC-34 cells; glutamate; free radicals; motor neurone disease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Shaw, PJ Univ Newcastle Upon Tyne, Sch Med, Dept Neurol, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England Univ Newcastle Upon Tyne Newcastle Upon Tyne Tyne & Wear England NE2 4HH
Citazione:
C.J. Eggett et al., "Development and characterisation of a glutamate-sensitive motor neurone cell line", J NEUROCHEM, 74(5), 2000, pp. 1895-1902

Abstract

Modification of the growth conditions of NSC-34 mouse neuroblastoma x motor neurone cells by serum depletion promotes the expression of functional glutamate receptors as the cells mature into a form that bears the phenotypiccharacterisation of motor neurones. Immunocytochemical studies demonstrated the presence of the glutamate receptor proteins NMDAR1, NMDAR2A/B, GluR1,GluR2, GluR2/3, GluR4, GluR6/7, and KA2. Toxicity assays using cell counting techniques demonstrated a mild but significant cell death (similar to 30%, p < 0.01) following a 24-h exposure to 1 mM glutamate that could be prevented by the presence of the glutamate receptor antagonists (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (10 mu M) and 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulphonamide (1 mu M). AS an indication of glutamate receptor functional activity a novel approach was used to detect the production of free radicals following stimulation with glutamate receptor agonists. The release of superoxide free radicalswas detected using a microelectrochemical sensor following addition of glutamate receptor agonists to the cell bathing solution. Alterations in intracellular calcium concentrations were examined using fura-2 imaging. Exposure of the differentiated NSC-34 cells to glutamate leads to an increase in intracellular calcium concentrations that is prevented by the presence of glutamate receptor antagonists. The motor neurone origin of these cells makesthem particularly useful for investigating the potential role of glutamatergic toxicity in motor neurone degeneration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:20:39