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Titolo:
Effect of p-chloroamphetamine on 5-HT1A and 5-HT7 serotonin receptor expression in rat brain
Autore:
Garcia-Osta, A; Frechilla, D; Del Rio, J;
Indirizzi:
Univ Navarra, Sch Med, Dept Pharmacol, Pamplona 31008, Spain Univ NavarraPamplona Spain 31008 Dept Pharmacol, Pamplona 31008, Spain
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 5, volume: 74, anno: 2000,
pagine: 1790 - 1797
SICI:
0022-3042(200005)74:5<1790:EOPO5A>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; MESSENGER-RNA EXPRESSION; NEUROTOXIN PARA-CHLOROAMPHETAMINE; TRANSCRIPTION FACTOR; ANTIDEPRESSANT TREATMENTS; GENE-EXPRESSION; BINDING SITES; MDMA ECSTASY; IN-VIVO; NEURONS;
Keywords:
p-chloroamphetamine; 5-hydroxytryptamine; 5-hydroxytryptamine(1A) receptors; 5-hydroxytryptamine(7) receptors; nuclear factor kappa B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Del Rio, J Univ Navarra, Sch Med, Dept Pharmacol, C Irunlarrea 1, Pamplona31008, Spain Univ Navarra C Irunlarrea 1 Pamplona Spain 31008 31008, Spain
Citazione:
A. Garcia-Osta et al., "Effect of p-chloroamphetamine on 5-HT1A and 5-HT7 serotonin receptor expression in rat brain", J NEUROCHEM, 74(5), 2000, pp. 1790-1797

Abstract

The aim of this study was to investigate if p-chloroamphetamine (PCA), which is neurotoxic to serotonin (5-HT) nerve terminals, was able to induce, like 3,4-methylenedioxymethamphetamine, a region-specific regulation of 5-HT1A receptor mRNA expression. The effect of PCA on the expression of 5-HT7 receptors, which share some pharmacological properties with 5-HT1A receptors, was comparatively studied. PCA (2 x 5 mg/kg) produced a lasting depletionof 5-HT content in the rat frontal cortex and hippocampus. In the hippocampus, the maximal 5-HT depletion was found on day 21 (-70%), whereas in the cortex, the highest 5-HT depletion was found on day 14 (-73%), with a partial but significant recovery on day 21. At the latter time point, 5-HT1A receptor mRNA expression was increased by 80% in the cortex and decreased by 50% in the hippocampus. The 5-HT1A receptor mRNA expression was also enhanced after exposure to PCA of rat cortical but not of hippocampal primary cultures. In regard to 5-HT7 receptor mRNA expression, the most remarkable change after PCA was the great increase (+200%) in the brainstem. Binding studies to 5-HT1A receptors matched the changes in receptor mRNA expression. Gelshift assays revealed enhanced nuclear protein binding to the kappa B sequence with use of cortical but not hippocampal extracts of PCA-treated rats. Overall, the data show region-specific changes in 5-HT receptor-type expression that may not be entirely dependent on the neurotoxic effect of PCA on5-HT terminals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:45:22