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Titolo:
Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vectors
Autore:
Feng, D; Nagy, JA; Brekken, RA; Pettersson, A; Manseau, EJ; Pyne, K; Mulligan, R; Thorpe, PE; Dvorak, HF; Dvorak, AM;
Indirizzi:
Beth Israel Deaconess Med Ctr, Dept Pathol, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 l, Boston, MA 02215 USA Harvard Med Sch, Boston, MA USA Harvard Med Sch Boston MA USAHarvard Med Sch, Boston, MA USA Harvard Med Sch, Harvard Inst Human Genet, Boston, MA USA Harvard Med SchBoston MA USA , Harvard Inst Human Genet, Boston, MA USA Childrens Hosp, Howard Hughes Med Inst, Boston, MA 02115 USA Childrens Hosp Boston MA USA 02115 Hughes Med Inst, Boston, MA 02115 USA Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Ctr, Dept Pharmacol, Dallas, TX 75235 USA Univ Texas, SW Med Ctr, Hamon Ctr Therapeut Oncol Res, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Therapeut Oncol Res, Dallas, TX 75235 USA
Titolo Testata:
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
fascicolo: 4, volume: 48, anno: 2000,
pagine: 545 - 555
SICI:
0022-1554(200004)48:4<545:ULOTVP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVASIVE BREAST-CANCER; TYROSINE KINASE; INHIBITING FACTORS; VEGF RECEPTOR-2; HUMAN PLACENTA; BLOOD-VESSELS; ANGIOGENESIS; CELLS; BINDING; IDENTIFICATION;
Keywords:
vascular permeability factor (VPF); vascular endothelial growth factor (VEGF); vascular permeability factor receptor (VPFR); vascular endothelial growth factor receptor (VEGFR); fetal liver kinase 1 (Flk-1); kinase insert domain-containing receptor (KDR); ultrastructure; immunocytochemistry; endothelial cells; tumor vessels; mouse kidney; vesiculovacuolar organelle (WO);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Dvorak, AM Beth Israel Deaconess Med Ctr, Dept Pathol, East Campus,330 Brookline Ave,Boston, MA 02215 USA Beth Israel Deaconess Med Ctr East Campus,330 Brookline Ave Boston MA USA 02215
Citazione:
D. Feng et al., "Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vectors", J HIST CYTO, 48(4), 2000, pp. 545-555

Abstract

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF)interacts with two high-affinity tyrosine kinase receptors, VEGFR-1 and VEGFR-2, to increase microvascular permeability and induce angiogenesis. Bothreceptors are selectively expressed by vascular endothelial cells and are strikingly increased in tumor vessels. We used a specific antibody to localize VEGFR-2 (FLK-1. KDR) in microvascular endothelium of normal mouse kidneys and in the microvessels induced by the TA3/St mammary tumor or by infection with an adenoviral vector engineered to express VPF/VEGF. A pre-embedding method was employed at the light and electron microscopic levels using either nanogold or peroxidase as reporters. Equivalent staining was observedon both the luminal and abluminal surfaces of tumor- and adenovirus-induced vascular endothelium, but plasma membranes at interendothelial junctions were spared except at sites connected to vesiculovacuolar organelles (VVOs). VEGFR-2 was also localized to the membranes and stomatal diaphragms of some VVOs. This staining distribution is consistent with a model in which VPF/VEGF increases microvascular permeability by opening VVOs to allow the transendothelial cell passage of plasma and plasma proteins.

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Documento generato il 05/07/20 alle ore 03:58:00