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Titolo:
Development and characterization of a tissue-engineered human oral mucosa equivalent produced in a serum-free culture system
Autore:
Izumi, K; Terashi, H; Marcelo, CL; Feinberg, SE;
Indirizzi:
Univ Michigan, Sch Dent, Dept Oral & Maxillofacial Surg, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 facial Surg, Ann Arbor, MI 48109 USA Univ Michigan, Med Ctr, Plast & Reconstruct Surg Sect, Ann Arbor, MI 48109USA Univ Michigan Ann Arbor MI USA 48109 ct Surg Sect, Ann Arbor, MI 48109USA
Titolo Testata:
JOURNAL OF DENTAL RESEARCH
fascicolo: 3, volume: 79, anno: 2000,
pagine: 798 - 805
SICI:
0022-0345(200003)79:3<798:DACOAT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL STEM-CELLS; IN-VIVO; HUMAN KERATINOCYTES; STRATUM-CORNEUM; DONOR AGE; EXPRESSION; INVITRO; DIFFERENTIATION; ACID; EPITHELIUM;
Keywords:
keratinocyte; acellular dermis; immunohistochemistry; essential fatty acid; oral mucosa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Feinberg, SE Univ Michigan, Sch Dent, Dept Oral & Maxillofacial Surg, B1-B204 UH,Box 0018,1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA Univ Michigan B1-B204 UH,Box 0018,1500 E Med Ctr Dr Ann Arbor MI USA 48109
Citazione:
K. Izumi et al., "Development and characterization of a tissue-engineered human oral mucosa equivalent produced in a serum-free culture system", J DENT RES, 79(3), 2000, pp. 798-805

Abstract

A problem maxillofacial surgeons face is a lack of sufficient autogenous oral mucosa for reconstruction of the oral cavity. Split-thickness or oral mucosa grafts require more than one surgical procedure and can result in donor site morbidity. Skin has disadvantages of adnexal structures and a different keratinization pattern than oral mucosa. In this study, we successfully assembled, ex vivo, a human oral mucosa equivalent, consisting of epidermal and dermal components, in a defined, essential-fatty-acid-deficient, serum-free culture medium without a feeder layer, that could be used for intra-oral grafting in humans. Autogenous oral keratinocytes were seeded onto a cadaveric dermis, AlloDerm(TM). The oral mucosa equivalent was cultured at an air-liquid interface for 2 wks. The resulting equivalent had a well-stratified parakeratinized epithelial layer similar to native oral keratinized mucosa. Expression of differentiation markers, filaggrin and cytokeratin 10/13, suggested a premature keratinized state. The presence of proliferationmarkers, proliferating cell nuclear antigen (PCNA) and Ki-67, suggested a state of hyperproliferation. Fatty acid composition of the equivalent was similar to that of in vitro cultured oral keratinocytes but differed from the that of in vivo native tissue, showing a lower content of 18:2 and 20:4, and a higher content of 16:1 and 18:1 fatty acids, respectively. The keratinocytes of the equivalent appeared to be in a more active and proliferativestate than native keratinized mucosa. The dynamic nature of the cell population on the oral mucosa equivalent may be beneficial for intra-oral grafting procedures and for transfection of the keratinocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:55:05