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Titolo:
Cytochemical demonstration of modification of carbohydrates in the mouse zona pellucida during folliculogenesis
Autore:
Aviles, R; El-Mestrah, M; Jaber, L; Castells, MT; Ballesta, J; Kan, FWK;
Indirizzi:
Queens Univ, Fac Hlth Sci, Sch Med, Dept Anat & Cell Biol, Kingston, ON K7L 3N6, Canada Queens Univ Kingston ON Canada K7L 3N6 Biol, Kingston, ON K7L 3N6, Canada Queens Univ, Fac Med, Dept Anat & Cell Biol, Kingston, ON K7L 3N6, Canada Queens Univ Kingston ON Canada K7L 3N6 Biol, Kingston, ON K7L 3N6, Canada Univ Murcia, Sch Med, Dept Cell Biol, Murcia, Spain Univ Murcia Murcia Spain Murcia, Sch Med, Dept Cell Biol, Murcia, Spain
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 3, volume: 113, anno: 2000,
pagine: 207 - 219
SICI:
0948-6143(200003)113:3<207:CDOMOC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
RICINUS-COMMUNIS AGGLUTININ; DATURA-STRAMONIUM LECTIN; ACETYL-D-GALACTOSAMINE; ULTRASTRUCTURAL-LOCALIZATION; LINKED OLIGOSACCHARIDES; MAMMALIAN FERTILIZATION; MONOCLONAL-ANTIBODIES; LOTUS-TETRAGONOLOBUS; BINDING-PROPERTIES; MAACKIA-AMURENSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Kan, FWK Queens Univ, Fac Hlth Sci, Sch Med, Dept Anat & Cell Biol, Kingston, ON K7L 3N6, Canada Queens Univ Kingston ON Canada K7L 3N6 gston, ON K7L 3N6, Canada
Citazione:
R. Aviles et al., "Cytochemical demonstration of modification of carbohydrates in the mouse zona pellucida during folliculogenesis", HISTOCHEM C, 113(3), 2000, pp. 207-219

Abstract

In the present study, lectin-gold cytochemistry and antibodies against ZP2and ZP3 glycoproteins were used to investigate the oligosaccharide contentof mouse ovarian zona pellucida (ZP) during follicular development. The entire thickness of the ZP and several organelles of the oocyte (cortical granules, Golgi apparatus, and vesicular aggregates) were reactive to RCA-I, DSA, AAA, WGA, MAA, and LFA throughout follicular development. HPA labeling was not detected at the earliest stages of follicular folliculogenesis. HPAreactivity was first observed in the ZP, Golgi apparatus, and the vesiclesof oocytes at the trilaminar primary follicle stage. HPA labeling in the ZP was always restricted to the inner region of the zona matrix. After neuraminidase treatment, HPA reacted with the entire ZP in ovarian follicles at different stages of development. Immunolabeling with specific antibodies showed that, although ZP2 and ZP3 glycoproteins were uniformly distributed inthe zona matrix of ovarian oocytes, there was a progressive increase in thickness of the ZP in parallel with the proliferation of follicular cells. ZP3 glycoprotein was also localized to the Golgi apparatus and vesicular aggregate. The present results suggest: (1) a difference in composition of carbohydrate content between the inner and outer region of the fully developedZP generated probably by a modification in the biosynthetic pathway of oligosaccharides in the oocyte during folliculogenesis, (2) that newly synthesized ZP glycoproteins displace previously synthesized ZP components in a direction toward the follicular cells and, therefore, no redistribution of the ZP matrix occurs during folliculogenesis, and (3) that the vesicular aggregates in the ooplasm constitute an intermediate step in the secretory pathway of ZP glycoproteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 00:34:14