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Titolo:
Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization
Autore:
van Gijlswijk, RPM; van de Corput, MPC; Bezrookove, V; Wiegant, J; Tanke, HJ; Raap, AK;
Indirizzi:
Leiden Univ, Dept Mol Cell Biol, Lab Cytochem & Cytometry, NL-2333 AL Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2333 AL NL-2333 AL Leiden, Netherlands
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 3, volume: 113, anno: 2000,
pagine: 175 - 180
SICI:
0948-6143(200003)113:3<175:SAPOHP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL AMPLIFICATION; DEPOSITION; DNA; PROBES; FISH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Raap, AK Leiden Univ, Dept Mol Cell Biol, Lab Cytochem & Cytometry, Wassenaarseweg 72, NL-2333 AL Leiden, Netherlands Leiden Univ Wassenaarseweg 72 Leiden Netherlands NL-2333 AL ands
Citazione:
R.P.M. van Gijlswijk et al., "Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization", HISTOCHEM C, 113(3), 2000, pp. 175-180

Abstract

A method is presented to conjugate horseradish peroxidase (HRP) to oligodeoxynucleotides for fluorescence in situ hybridization assays employing tyramide signal amplification (TSA). HRP is covalently bound to the oligonucleotide by thiol ether linkage and purified by high-performance liquid chromatography. With TSA detection, a single HRP-labeled oligonucleotide probe is sufficient for in situ detection of clustered DNA repeat sequences with a degree of repetition between 20 and 50.

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Documento generato il 28/11/20 alle ore 15:39:48