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Titolo:
Analysis of Leptospira isolates from mainland Portugal and the Azores islands
Autore:
Collares-Pereira, M; Korver, H; Thi, BVC; Santos-Reis, M; Bellenger, E; Baranton, G; Terpstra, WJ;
Indirizzi:
UNL, Inst Higiene & Med Trop, P-1349008 Lisbon, Portugal UNL Lisbon Portugal P-1349008 ene & Med Trop, P-1349008 Lisbon, Portugal Royal Trop Inst, NL-1105 AZ Amsterdam, Netherlands Royal Trop Inst Amsterdam Netherlands NL-1105 AZ Amsterdam, Netherlands Inst Pasteur, F-75724 Paris, France Inst Pasteur Paris France F-75724Inst Pasteur, F-75724 Paris, France Ctr Biol Ambiental UL, P-1700 Lisbon, Portugal Ctr Biol Ambiental UL Lisbon Portugal P-1700 UL, P-1700 Lisbon, Portugal
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 2, volume: 185, anno: 2000,
pagine: 181 - 187
SICI:
0378-1097(20000415)185:2<181:AOLIFM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESTRICTION-ENDONUCLEASE ANALYSIS; MONOCLONAL-ANTIBODIES; SEROVAR; INTERROGANS; CLASSIFICATION; POLYMORPHISMS; SEROGROUP; PCR;
Keywords:
Portugal; taxonomy; serotyping; genotyping; Leptospira;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Collares-Pereira, M UNL, Inst Higiene & Med Trop, R da Junqueira 96, P-1349008 Lisbon, Portugal UNL R da Junqueira 96 Lisbon Portugal P-1349008 tugal
Citazione:
M. Collares-Pereira et al., "Analysis of Leptospira isolates from mainland Portugal and the Azores islands", FEMS MICROB, 185(2), 2000, pp. 181-187

Abstract

From 228 recent Leptospira isolates from mainland Portugal and Azorean wild mammals, 149 were characterized at the serovar level by monoclonal antibodies (MAbs), a quick serological method in epidemiological studies. In order to compare this antigenic information with that from new genetic techniques, a sample of isolates was analyzed through pulsed-field agarose gel electrophoresis (PFGE) (n = 71), mapped restriction site polymorphisms (MRSPs) in PCR-amplified rRNA genes (n = 45, including 13 saprophytes) and arbitrarily primed polymerase chain reaction (AP-PCR) fingerprinting (n = 32). MRSPand AP-PCR lead to species identification of the studied 32 pathogenic isolates: Leptospira interrogans (n = 3), Leptospira borgpetersenii (n = 8) and Leptospira bougpetersenii (n = 21). MAbs and PFGE characterized pathogenic isolates at the serovar level and resulted mainly in agreement (64%) although many discrepancies (35%) were observed. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 22:08:06