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Titolo:
N-cadherin function is required for differentiation-dependent cytoskeletalreorganization in lens cells in vitro
Autore:
Ferreira-Cornwell, MC; Veneziale, RW; Grunwald, GB; Menko, AS;
Indirizzi:
Thomas Jefferson Univ, Dept Pathol Anat & Cell Biol, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 256, anno: 2000,
pagine: 237 - 247
SICI:
0014-4827(20000410)256:1<237:NFIRFD>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR ADHERENS JUNCTIONS; CYTOPLASMIC CONTROL; ADHESION MOLECULES; CHICK DEVELOPMENT; 135-KD RECEPTOR; A-CAM; EXPRESSION; ANTIBODY;
Keywords:
cadherins; catenins; lens; development; differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Menko, AS Thomas Jefferson Univ, Dept Pathol Anat & Cell Biol, 1020 LocustSt, Philadelphia, PA 19107 USA Thomas Jefferson Univ 1020 Locust St Philadelphia PA USA 19107 A
Citazione:
M.C. Ferreira-Cornwell et al., "N-cadherin function is required for differentiation-dependent cytoskeletalreorganization in lens cells in vitro", EXP CELL RE, 256(1), 2000, pp. 237-247

Abstract

Members of the cadherin family of cell adhesion molecules participate in calcium-dependent cell-cell adhesions that are necessary for the cell sorting events that regulate early developmental processes. Although individual cadherin molecules have been shown to participate in tissue histogenesis, the regulation of function of these receptors in cell differentiation has been more difficult to identify. We have determined that N-cadherin linkage tothe cytoskeleton is correlated with lens cell differentiation in vivo. Through the use of a chick embryo lens culture system that mimics differentiation in vivo, we have determined that N-cadherin linkage to the cytoskeletonis altered and lens differentiation is blocked by function-blocking antibodies to N-cadherin, In the presence of the N-cadherin function-blocking antibody, NCD-2, both N-cadherin and filamentous actin are prevented from organizing at the cortical membranes. This correlates with an inhibition of lens morphogenesis and differentiation. These results are paralleled by changes in the expression of the molecular components of the cadherin-catenin complex and their linkage to the actin cytoskeleton. In the presence of NCD-2,expression of N-cadherin, alpha-catenin, and beta-catenin is inhibited andtheir association with the cytoskeleton blocked, Overall cadherin expression, however, remains unchanged as demonstrated by studies with a pan-cadherin antibody. This is accompanied by an increase in expression of the cadherin cytoskeletal protein plakoglobin. Although the cells have tried to compensate for the loss of N-cadherin by up-regulation of another cadherin(s) and plakoglobin, this is unable to compensate for N-cadherin function. The data strongly suggest that N-cadherin and its associated cytoskeleton play animportant role in the differentiation process that leads to the formation of the crystalline lens. (C) 2000 Academic Press.

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Documento generato il 19/09/20 alle ore 17:16:00