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Titolo:
Preferential expression of NuMA in the nuclei of proliferating cells
Autore:
Taimen, P; Viljamaa, N; Kallajoki, M;
Indirizzi:
Univ Turku, Dept Pathol, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 Dept Pathol, FIN-20520 Turku, Finland Turku Grad Sch Biomed Sci, Turku, Finland Turku Grad Sch Biomed Sci Turku Finland Sch Biomed Sci, Turku, Finland
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 256, anno: 2000,
pagine: 140 - 149
SICI:
0014-4827(20000410)256:1<140:PEONIT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOTIC APPARATUS PROTEIN; MONOCLONAL-ANTIBODY; MAMMALIAN-CELLS; MATRIX PROTEIN; KI-67 DETECTS; COILED-COIL; SPN ANTIGEN; PTK2 CELLS; APOPTOSIS; SPINDLE;
Keywords:
NuMA; nuclear matrix; interphase nucleus; tissues; differentiation; proliferation; Ki-67 antigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kallajoki, M Univ Turku, Dept Pathol, Kiinamyllynkatu 10, FIN-20520 Turku,Finland Univ Turku Kiinamyllynkatu 10 Turku Finland FIN-20520 inland
Citazione:
P. Taimen et al., "Preferential expression of NuMA in the nuclei of proliferating cells", EXP CELL RE, 256(1), 2000, pp. 140-149

Abstract

Nuclear mitotic apparatus protein (NuMA) has an indispensable function in normal mitosis as an organizer of the mitotic spindle. NuMA is a prominent component of interphase cell nuclear matrix but its role during interphase is largely unknown. We examined the presence of NuMA in several human tissues. The majority of cells were positive for NuMA but a few negative cell types were found, including spermatozoa, superficial keratinocytes, neutrophil granulocytes, syncytiotrophoblasts, and some neurons, fibro blasts, and smooth and skeletal muscle cells. We further investigated the presence of NuMA in a cultured estrogen-dependent human breast cancer cell Line and observed the disappearance of nuclear NuMA in the quiescent cells. The percentage of NuMA-positive cells diminished from an initial similar to 100 to 60% during 6 days of culture. The presence of NuMA correlated positively with the presence of proliferation marker Ki-67 antigen and negatively with the culture time, confluence, and size of the cell islets. These results show that some nonproliferating, highly differentiated cell types lack NuMA and that cells may lose their NuMA without dramatic effects on the nuclear shape. This suggests that NuMA may be a nonessential component of the interphase nucleus. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 19:11:16