Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Inducible nitric oxide synthase (iNOS) expression in liver and splenic T lymphocyte rise are associated with liver histological damage during experimental hepatitis A virus (HAV) infection in Callithrix jacchus
Autore:
Pinto, MA; Marchevsky, RS; Pelajo-Machado, M; Santiago, MA; Pissurno, JW; Franca, MS; Baptista, ML; Gouvea, AS; Santana, AA; Bertho, AL; Schatzmayr, HG; Gaspar, AMC; Kubelka, CF;
Indirizzi:
FIOCRUZ, IOC, Dept Virol, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ Rio De Janeiro Brazil BR-21045900 BC45900 Rio De Janeiro, Brazil FIOCRUZ, IOC, Dept Pathol, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ RioDe Janeiro Brazil BR-21045900 BC45900 Rio De Janeiro, Brazil FIOCRUZ, IOC, Dept Protozool, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ Rio De Janeiro Brazil BR-21045900 BC45900 Rio De Janeiro, Brazil FIOCRUZ, IOC, Biomanguinhos, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ Rio De Janeiro Brazil BR-21045900 BC45900 Rio De Janeiro, Brazil
Titolo Testata:
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
fascicolo: 1, volume: 52, anno: 2000,
pagine: 3 - 10
SICI:
0940-2993(200003)52:1<3:INOS(E>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
A VIRUS; VIRAL-HEPATITIS; MECHANISMS; DEFENSE; INJURY;
Keywords:
nitric oxide synthase, inducible; T-lymphocyte; liver, T-lymphocyte; spleen, T-lymphocyte; hepatitis A virus infection; Callithrix jacchus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Pinto, MA FIOCRUZ, IOC, Dept Virol, Av Brazil 4365, BR-21045900 Rio De Janeiro, Brazil FIOCRUZ Av Brazil 4365 Rio De Janeiro Brazil BR-21045900 BCazil
Citazione:
M.A. Pinto et al., "Inducible nitric oxide synthase (iNOS) expression in liver and splenic T lymphocyte rise are associated with liver histological damage during experimental hepatitis A virus (HAV) infection in Callithrix jacchus", EXP TOX PAT, 52(1), 2000, pp. 3-10

Abstract

Callithrix jacchus is considered a reliable animal model for hepatitis A virus (HAV) infection. All three HAV orally inoculated marmosets developed hepatitis - the infection was monitored by continuous virus shedding, high levels of serum enzyme alanine aminotransferase, specific antibody and seroconversion 3-6 weeks after HAV inoculation. HAV antigen was detected in liver by immunofluorescence 4 days post inoculation (PI) and onwards. To gain insight into the biological role of inducible nitric oxide synthase (iNOS) during immune-related acute liver injury the enzyme was searched in frozen biopsies; immunofluorescent labeling was found in the cytoplasm of liver cells mainly Kupffer's cells and spleen macrophages (CD68+) starting 11 days PI with maximum intensity on the fifth to sixth week PI. Necroinflammatory liver lesions characteristic of viral hepatitis were also observed at 10 days PI with maximum severity at 4 to 6 weeks PI. Furthermore, T lymphocytes (CD2+) were raised at this time point. No difference was evident in the frequency of B lymphocytes (CD20+). Therefore, iNOS expression preceded necroinflammatory liver lesion and maximal immunofluorescence reaction was coincident with tissue injury, supporting the hypothesis that NO contributes to hepatic cytotoxic mechanism but also to virus clearance. The concomitant risein T-lymphocyte population may suggest a role for these cells in this and/or other independent HAV-induced pathological changes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/06/20 alle ore 15:53:01