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Titolo:
Characterization of the functionally related sites in the neural inducing gene noggin
Autore:
Liu, WD; Ren, CP; Shi, JL; Feng, XL; He, ZW; Xu, LG; Lan, K; Xie, L; Peng, Y; Fan, J; Kung, HF; Yao, KT; Xu, RH;
Indirizzi:
Hunan Med Univ, Canc Res Inst, Changsha 410078, Hunan, Peoples R China Hunan Med Univ Changsha Hunan Peoples R China 410078 nan, Peoples R China NCI, Frederick Canc Res & Dev Ctr, Basic Res Lab, Metab & Canc SusceptibilSect,NIH, Frederick, MD 21702 USA NCI Frederick MD USA 21702 c SusceptibilSect,NIH, Frederick, MD 21702 USA NICHHD, Lab Cellular & Mol Biophys, NIH, Bethesda, MD 20892 USA NICHHD Bethesda MD USA 20892 r & Mol Biophys, NIH, Bethesda, MD 20892 USA Univ Hong Kong, Inst Mol Biol, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Hong Kong Hong Kong Peoples R China Kong, Peoples R China First Mil Med Univ, Dept Pathol, Guangzhou 510515, Peoples R China First Mil Med Univ Guangzhou Peoples R China 510515 515, Peoples R China WiCell Res Inst, Madison, WI 53705 USA WiCell Res Inst Madison WI USA 53705 Cell Res Inst, Madison, WI 53705 USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 270, anno: 2000,
pagine: 293 - 297
SICI:
0006-291X(20000402)270:1<293:COTFRS>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL INDUCTION; SPEMANN ORGANIZER; XENOPUS ECTODERM; KUNITZ DOMAIN; INHIBITION; EMBRYOS; RECEPTOR; SIGNALS; BMP-4; NEURALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Yao, KT Hunan Med Univ, Canc Res Inst, Changsha 410078, Hunan, Peoples R China Hunan Med Univ Changsha Hunan Peoples R China 410078 ples R China
Citazione:
W.D. Liu et al., "Characterization of the functionally related sites in the neural inducing gene noggin", BIOC BIOP R, 270(1), 2000, pp. 293-297

Abstract

Previously we have shown that blocking bone morphogenetic protein (BMP) receptor signaling by a dominant negative BMP receptor causes neurogenesis inXenopus animal caps (ACs), whereas the physiological neural inducer nogginacts as a homodimer physically binding to BMP-4 and disrupting its signaling at the ligand level. The present study attempted to elucidate the relationship between the structure and function of noggin. By replacing some cysteine residues with serine residues through a site-directed mutagenesis strategy, we generated three noggin mutants, C145S, C205S, and C(218, 220, 222)S (3CS). Although mRNAs encoded by these mutants were translated as efficiently as wild-type (WT) noggin mRNA, they behaved differently when expressedin vivo. Expression of WT noggin or C205S in Xenopus ACs converted the explants (prospective ectoderm) into neural tissue, indicated by the neural-like morphology and expression of the pan neural marker NCAM in the ACs. In contrast, ACs expressing C145S or 3CS sustained an epidermal fate like the control caps. Similar results were observed in the mesoderm where C205S (butnot C145S and 3CS) displayed dorsalizing activity as well as WT noggin. Altogether, our results suggest that Cys145 alone or Cys(218, 220, 222) as a whole in noggin protein is required for the biological activities of noggin, probably participating in the dimerization of noggin with BMP-4 or itself. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:00:05