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Titolo:
Role of a glycocalyx on coronary arteriole permeability to proteins: evidence from enzyme treatments
Autore:
Huxley, VH; Williams, DA;
Indirizzi:
Univ Missouri, Sch Med, Dept Physiol, Columbia, MO 65212 USA Univ Missouri Columbia MO USA 65212 Dept Physiol, Columbia, MO 65212 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 4, volume: 278, anno: 2000,
pagine: H1177 - H1185
SICI:
0363-6135(200004)278:4<H1177:ROAGOC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
FROG MESENTERIC CAPILLARIES; ENDOTHELIAL-CELL GLYCOCALYX; FILTRATION COEFFICIENT; RABBIT HEART; ALBUMIN; ULTRASTRUCTURE; VESSELS; PLASMA; MACROMOLECULES; MICROSCOPY;
Keywords:
porcine serum albumin; alpha-lactalbumin; microspectrofluorometry; microcirculation; endothelium; Yucatan miniature swine; protease; heparinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Huxley, VH Univ Missouri, Sch Med, Dept Physiol, Columbia, MO 65212 USA Univ Missouri Columbia MO USA 65212 ol, Columbia, MO 65212 USA
Citazione:
V.H. Huxley e D.A. Williams, "Role of a glycocalyx on coronary arteriole permeability to proteins: evidence from enzyme treatments", AM J P-HEAR, 278(4), 2000, pp. H1177-H1185

Abstract

Whereas the glycocalyx of endothelial cells has been shown to influence solute flux from capillary microvessels, little is known about its contribution to the movement of macromolecules across the walls of other microvessels. We evaluated the hypothesis that a glycocalyx contributes resistance to protein flux measured in coronary arterioles. Apparent solute permeability (P-s) to two proteins of different size and similar charge, alpha-lactalbumin (alpha-lactalb) and porcine serum albumin (PSA), was determined in arterioles isolated from the hearts of 43 female Yucatan miniature swine. P-s wasassessed in arterioles with an "intact" glycocalyx under control conditions and again after suffusion with adenosine (Ado, 10(-5) M, n = 42 arterioles, N = 29 pigs). In a second set of experiments (n = 21 arterioles, N = 21 pigs) arteriolar P-s was determined before and after perfusion with enzyme (pronase or heparinase), which was used to digest the glycocalyx. P-s was assessed a third time on those microvessels after exposure to Ado. Consistent with the hypothesis, P-s for PSA (p(s)(PSA)) and P-s for alpha-lactalb (P-s(alpha-)lactalb) increased from basal levels following enzyme treatment. Subsequent suffusion with Ado, a significant metabolite known to alter coronary vascular smooth muscle tone and permeability, resulted in a significant reduction of basal P-s(alpha-lactalb) in, both untreated and enzyme-treated arterioles. Furthermore, in untreated arterioles, p(s)(PSA) was unchanged by Ado suffusion, whereas Ado induced a pronounced reduction in p(s)(PSA)of enzyme-treated vessels. These data demonstrate that in intact coronary arterioles an enzyme-sensitive layer, most likely at the endothelial cell surface, contributes significantly to net barrier resistance to solute flux.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 18:11:22