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Titolo:
Inhibition of angiogenesis and vascular tumor growth by interferon-producing cells - A gene therapy approach
Autore:
Albini, A; Marchisone, C; Del Grosso, F; Benelli, R; Masiello, L; Tacchetti, C; Bono, M; Ferrantini, M; Rozera, C; Truini, M; Belardelli, F; Santi, L; Noonan, DM;
Indirizzi:
Ist Nazl Ric Canc, Ctr Biotecnol Avanzate, Mol Biol Lab, I-16132 Genoa, Italy Ist Nazl Ric Canc Genoa Italy I-16132 Mol Biol Lab, I-16132 Genoa, Italy Ist Nazl Ric Canc, Ctr Biotecnol Avanzate, Tumor Progress Sect, I-16132 Genoa, Italy Ist Nazl Ric Canc Genoa Italy I-16132 rogress Sect, I-16132 Genoa, Italy Univ Genoa, Anat Sect, Dept Expt Med, Genoa, Italy Univ Genoa Genoa Italy iv Genoa, Anat Sect, Dept Expt Med, Genoa, Italy Ist Super Sanita, I-00161 Rome, Italy Ist Super Sanita Rome Italy I-00161 st Super Sanita, I-00161 Rome, Italy Osped San Martino Genova, Serv Anat Istol & Citol Patol, Genoa, Italy Osped San Martino Genova Genoa Italy Istol & Citol Patol, Genoa, Italy
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 4, volume: 156, anno: 2000,
pagine: 1381 - 1393
SICI:
0002-9440(200004)156:4<1381:IOAAVT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECONSTITUTED BASEMENT-MEMBRANE; ENDOTHELIAL-CELLS; ANTIANGIOGENIC AGENTS; GELATINASE PRODUCTION; IN-VIVO; BETA; EXPRESSION; ALPHA; CANCER; LINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Noonan, DM Ist Nazl Ric Canc, Ctr Biotecnol Avanzate, Mol Biol Lab, I-16132 Genoa, Italy Ist Nazl Ric Canc Genoa Italy I-16132 b, I-16132 Genoa, Italy
Citazione:
A. Albini et al., "Inhibition of angiogenesis and vascular tumor growth by interferon-producing cells - A gene therapy approach", AM J PATH, 156(4), 2000, pp. 1381-1393

Abstract

We developed an in vivo gene therapy approach to characterize and optimizethe anti-angiogenic activity of class I interferons (IFNs), using packaging cell lines producing an amphotropic LXSN-based retrovirus expressing either IFN-alpha 1 (alpha 1Am21), IFN-beta (beta Am12) murine cDNAs, or the vector alone (neoAm12). Pretreatment of endothelial-like Eahy926 cells in vitro with conditioned media (CM) from alpha 1Am12 or beta Am12 cells for 48 hours significantly inhibited their migration and invasion as compared to neoAm12-CM-treated cells. beta Am12-CM also inhibited the formation of capillary-like structures on Matrigel by EAhy926 cells. In vivo, inclusion of the beta Am12 cells strongly inhibited, and alpha 1Am12 partially inhibited, the angiogenic response in the Matrigel sponge model in both immune-competentand athymic nude mice. Electron microscopy showed a reduction of host cellinfiltration in alpha 1Am12- and beta Am12-containing sponges and reduction of invading tubular clefts of host cells as compared to controls. Finally, inoculation of either alpha 1Am12 or beta Am12 cells (10%) along with a highly angiogenic Kaposi's sarcoma cell Line (90%) resulted in a powerful reduction of tumor growth in nude mice in vivo, as did infection with the interferon-alpha-producing retroviruses. These data suggest that a gene therapy approach using class I interferons can effectively inhibit tumor angiogenesis and growth of vascular tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 20:54:35