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Titolo:
International multi-centre evaluation of a dipstick assay for human leptospirosis
Autore:
Smits, HL; Hartskeerl, RA; Terpstra, WJ;
Indirizzi:
Univ Amsterdam, Acad Med Ctr, Royal Trop Inst, Dept Biomed Res, NL-1105 AZAmsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1105 AZ AZAmsterdam, Netherlands
Titolo Testata:
TROPICAL MEDICINE & INTERNATIONAL HEALTH
fascicolo: 2, volume: 5, anno: 2000,
pagine: 124 - 128
SICI:
1360-2276(200002)5:2<124:IMEOAD>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; ELISA;
Keywords:
leptospirosis; dipstick; serodiagnosis; microscopic agglutination test; ELISA; sensitivity; specificity; IgM response; haemorrhagic fever;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Smits, HL Univ Amsterdam, Acad Med Ctr, Royal Trop Inst, Dept Biomed Res, Meibergdreef 39, NL-1105 AZ Amsterdam, Netherlands Univ Amsterdam Meibergdreef 39 Amsterdam Netherlands NL-1105 AZ
Citazione:
H.L. Smits et al., "International multi-centre evaluation of a dipstick assay for human leptospirosis", TR MED I H, 5(2), 2000, pp. 124-128

Abstract

A dipstick assay for the detection of Leptospira-specific immunoglobulin M(IgM) antibodies in human sera was evaluated in 27 laboratories in 23 countries. 873 serum samples from 711 patients including 329 laboratory-confirmed leptospirosis case patients, 239 noncase patients and 69 patients with viral infections causing heamorrhagic fever were tested. Relative to the results of the reference leptospirosis test, the sensitivity of the dipstick assay was 84.5% for serum samples collected during the first 10 days of the disease and 92.1% for serum samples collected 10-30 days after the onset ofdisease. The specificity was 87.5% and 94.4%, respectively Similar to viral haemorrhagic fevers, leptospirosis may cause bleeding. A small number of serum samples from patients with haemorrhagic viral infections gave a weak (1 +) stain. All other samples were negative. In conclusion, the dipstick assay is sensitive and specific and reacts well with serum samples from patients infected with a range of leptospiral strains. It is also easy to use and does not require special equipment or refrigeration. Therefore the assayis ideal for use in developing countries and rural settings.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:36:01