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Titolo:
Transposon mutagenesis in Halomonas eurihalina
Autore:
Llamas, I; Argandona, M; Quesada, E; del Moral, A;
Indirizzi:
Univ Granada, Fac Pharm, Dept Microbiol, Exopolysaccharide Res Grp, Granada 18071, Spain Univ Granada Granada Spain 18071 accharide Res Grp, Granada 18071, Spain
Titolo Testata:
RESEARCH IN MICROBIOLOGY
fascicolo: 1, volume: 151, anno: 2000,
pagine: 13 - 18
SICI:
0923-2508(200001/02)151:1<13:TMIHE>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRAM-NEGATIVE BACTERIA; MODERATELY HALOPHILIC EUBACTERIA; VOLCANIELLA-EURIHALINA; CHROMOSOMAL INSERTION; HOST-RANGE; DERIVATIVES; RESISTANCE; PLASMIDS; TN5; DNA;
Keywords:
halophile; transposon; mutagenesis; Halomonas eurihalina; conjugation; exopolysaccharide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: del Moral, A Univ Granada, Fac Pharm, Dept Microbiol, Exopolysaccharide Res Grp, CampusUniv Cartuja, Granada 18071, Spain Univ Granada Campus Univ Cartuja Granada Spain 18071 , Spain
Citazione:
I. Llamas et al., "Transposon mutagenesis in Halomonas eurihalina", RES MICROB, 151(1), 2000, pp. 13-18

Abstract

We have established a transposon mutagenesis procedure for the moderate halophile Halomonas eurihalina, a bacteria that produces an exopolysaccharide(EPS) of considerable biotechnological interest. We used suicide plasmids pUT and pSUP102 to introduce the transposons mini-Tn5 and Tn1732 into H. eurihalina via Escherichia coli mediated conjugation. Southern hybridization analysis demonstrated that insertions of the transposon mini-Tn5 into H. eurihalina occurred randomly at single sites in the chromosome, whereas Tn1732 insertion also took place at random, but simultaneously, at several sites. Phenotypic analysis revealed that different mutants were generated by using mini-Tn5. The isolation of exopolysaccharide-defective strains is the first stage towards carrying out genetic studies on EPS production by this microorganism. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 12:48:42