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Titolo:
Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells
Autore:
Velazhahan, R; Samiyappan, R; Vidhyasekaran, P;
Indirizzi:
Tamil Nadu Agr Univ, Dept Plant Pathol, Coimbatore 641003, Tamil Nadu, India Tamil Nadu Agr Univ Coimbatore Tamil Nadu India 641003 Tamil Nadu, India
Titolo Testata:
PHYTOPARASITICA
fascicolo: 2, volume: 28, anno: 2000,
pagine: 131 - 139
SICI:
0334-2123(2000)28:2<131:POAEAC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORYZA-SATIVA L; RHIZOCTONIA-SOLANI; BASIC CHITINASE; FUNGAL GROWTH; GENE; RESISTANCE; EXPRESSION; TOBACCO; PLANTS; BETA-1,3-GLUCANASE;
Keywords:
basic chitinase; hyphal lysis; Oryza sativa; Rhizoctonia solani; sheath blight;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Velazhahan, R Tamil Nadu Agr Univ, Dept Plant Pathol, Coimbatore 641003, Tamil Nadu, India Tamil Nadu Agr Univ Coimbatore Tamil Nadu India 641003 ndia
Citazione:
R. Velazhahan et al., "Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells", PHYTOPARASI, 28(2), 2000, pp. 131-139

Abstract

Suspension-cultured rice cells showed an appreciable amount of chitinase activity when the cultured cells were treated with an elicitor isolated fromRhizoctonia solani, the rice sheath blight pathogen. A fivefold increase in chitinase activity was observed 24 h after elicitor treatment. The elicitor-inducible chitinase was purified by ammonium sulfate fractionation, chitin affinity chromatography and gel filtration. Its molecular weight is 35 kDa and it has an isoelectric point of 8.3. The 35 kDa basic chitinase inhibited mycelial growth of R. solani bz vitro. Morphological changes appeared within 1 h following exposure of mycelium to the chitinase. The hyphal tip showed marked swelling and subsequently lysis was also observed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:11:40