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Titolo:
Extracellular matrix components in retrocorneal fibrous membrane in comparison to corneal endothelium and Descemet's membrane
Autore:
Leung, EW; Rife, L; Smith, RE; Kay, EP;
Indirizzi:
Univ So Calif, Doheny Eye Inst, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 ye Inst, Los Angeles, CA 90033 USA Univ So Calif, Keck Sch Med, Dept Ophthalmol, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 thalmol, Los Angeles, CA 90033 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 4, volume: 6, anno: 2000,
pagine: 15 - 23
SICI:
1090-0535(20000307)6:4<15:EMCIRF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
IV COLLAGEN; SPLICE VARIANTS; VIII COLLAGEN; XII COLLAGEN; CELL-SHAPE; BETA-IG; EXPRESSION; MODULATION; LEUKOCYTES; TISSUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Kay, EP Univ So Calif, Doheny Eye Inst, 1450 San Pablo St DVRC 203, Los Angeles, CA 90033 USA Univ So Calif 1450 San Pablo St DVRC 203 Los Angeles CAUSA 90033
Citazione:
E.W. Leung et al., "Extracellular matrix components in retrocorneal fibrous membrane in comparison to corneal endothelium and Descemet's membrane", MOL VIS, 6(4), 2000, pp. 15-23

Abstract

PURPOSE: To investigate the extracellular matrix macromolecules found in Descemet's membrane and in retrocorneal fibrous membrane (RCFM), and to examine whether the corneal endothelium has the capacity to produce both basement and non-basement membrane phenotypes. METHODS: Rabbit corneas with and without RCFM were analyzed by immunofluorescence using antibodies to 8 different collagens (basement membrane collagens: types IV and VIII; fibrillar collagens: types I and III; interfibrillar collagens: type VI and two spliced variant forms of type XII and one anchoring fiber: type VII), proteoglycans (perlecan and decorin), beta ig-h3 and laminin-1. RESULTS: Normal corneal endothelium stains positively for all of the tested collagen types except type VII collagen. On the other hand, Descemet's membrane reacts positively only to the type IV collagen antibody. When non-collagenous components in normal cornea were examined, corneal endothelium stained positively for perlecan, decorin, beta ig-h3 and laminin, whereas Descemet's membrane staining for these proteins was negative. When collagenouscomponents of RCFM were examined, RCFM stained positively for all of the tested collagen types except type IV collagen. When non-collagneous components of RCFM were examined, RCFM demonstrated a strong positive staining withdecorin, beta ig-h3 and laminin, while perlecan staining was weak. CONCLUSIONS: These observations suggest that corneal endothelium is able to produce both basement membrane phenotypes and non-basement membrane, fibrillar phenotypes. This in vivo study confirms our in vitro model of endothelial mesenchymal transformation, in which corneal endothelial cells are transformed to fibroblasts that are responsible for fibrosis.

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Documento generato il 23/01/21 alle ore 03:17:10