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Titolo:
Induction of experimental proteinuria in vivo following infusion of human plasma hemopexin
Autore:
Cheung, PK; Klok, PA; Baller, JFW; Bakker, WW;
Indirizzi:
Univ Groningen, Dept Pathol & Lab Med, Groningen, Netherlands Univ Groningen Groningen Netherlands & Lab Med, Groningen, Netherlands
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 4, volume: 57, anno: 2000,
pagine: 1512 - 1520
SICI:
0085-2538(200004)57:4<1512:IOEPIV>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MINIMAL CHANGE NEPHROPATHY; NEPHROTIC SYNDROME; GLOMERULAR-PERMEABILITY; LIPOID NEPHROSIS; ANIONIC SITES; ALBUMINURIA; RATS;
Keywords:
hemopexin; proteinuria; minimal change disease; idiopathic nephrotic syndrome; transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Bakker, WW Univ Groningen Hosp, Dept Pathol & Lab Med, POB 30-001, NL-9700RB Groningen, Netherlands Univ Groningen Hosp POB 30-001 Groningen Netherlands NL-9700 RB
Citazione:
P.K. Cheung et al., "Induction of experimental proteinuria in vivo following infusion of human plasma hemopexin", KIDNEY INT, 57(4), 2000, pp. 1512-1520

Abstract

Background. The human plasma constituent hemopexin (Hx), following incubation with renal tissue, is able to induce glomerular alterations in vitro that are similar to those seen in minimal change disease (MCD). Whether this acute phase reactant is also able to induce proteinuria and minimal change-like alterations in vivo is questioned. Methods. In the first set of experiments, Hx (4.0 mg in 5.0 mt saline) or equal amounts of control fraction. that is. heat-inactivated Hx (HI-Hx). were infused into conscious rats (N = 6) that had been surgically equipped with a cannula inserted into the suprarenal artery (SRA), enabling direct contact of the infusate and the renal microvasculature. Each animal received HI-Hx at day 1 for 15 minutes (flow rate 20.0 mL/h), subsequently followed by saline for seven hours (Flow rate 5.0 mL/h), after which the cannula was disconnected. At day 2, identical infusions in the same rat were carried out, using native Hx. Urine samples collected every 30 minutes during the experi ments were monitored for protein content using standard methods. In thesecond set of experiments, unilateral perfusion was done ex vivo in anesthetized rats with Hx (N = 5) or HI-Hx (N = 3; 15 mg/mL; 4.0 mt during 6 min). After reconnection of the circulation. urine samples of both kidneys werecollected every 30 minutes during five hours via ureter cannulation. Urinary protein (expressed as the difference in excretion between perfused and nonperfused kidney) was calculated in mg/24 h. In additional experiments, rats were sacrificed two hours after perfusion of Hx or heat-inactivated (control) Hx (first set of experiments) or after five hours (second set of experiments), and kidneys were processed for immunohistochemical and ultrastructural examination. Results. The results of experiment 1 show a significant increase of proteinuria after Hx infusion versus HI-Hx (means +/- SD. 41.91 +/- 16.01 mg/24 hvs. control, 21.22 +/- 5.69 mg/24 h; P less than or equal to 0.03). Histochemical and electron microscopical examination of kidney tissue fragments taken at the time of proteinuria showed diminished expression of glomerular ecto-ATPase and enhanced effacement of epithelial foot processes at the ultrastructural level. In the second set of experiments, the results show significant urinary protein excretion peaking one hour after perfusion (6.63 +/- 7.06 mg/24 h), exclusively in Hx-perfused animals (analysis of variance, P less than or equal to 0.001). Conclusion. It is concluded that Hx is able to induce proteinuria associated with MCD-like alterations in rat kidney in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:30:42