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Titolo:
Three-dimensional organisation of mitochondrial clusters in regenerating dorsal root ganglion (DRG) neurons from neonatal rats: evidence for mobile mitochondrial pools
Autore:
Dedov, VN; Armati, PJ; Roufogalis, BD;
Indirizzi:
Univ Sydney, Dept Pharm, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 Pharm, Sydney, NSW 2006, Australia Univ Sydney, Sch Biol Sci, Neurosci Unit, Sydney, NSW 2006, Australia UnivSydney Sydney NSW Australia 2006 i Unit, Sydney, NSW 2006, Australia
Titolo Testata:
JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM
fascicolo: 1, volume: 5, anno: 2000,
pagine: 3 - 10
SICI:
1085-9489(200003)5:1<3:TOOMCI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSPORT; KINESIN; JC-1; TRANSIENTS; FILOPODIA; POLARITY; DYNAMICS; CELLS;
Keywords:
dorsal root ganglion neurons; mitochondria; mitochondrial clusters; neuronal regeneration; axonal transport; neuronal polarization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Roufogalis, BD Univ Sydney, Dept Pharm, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 NSW 2006, Australia
Citazione:
V.N. Dedov et al., "Three-dimensional organisation of mitochondrial clusters in regenerating dorsal root ganglion (DRG) neurons from neonatal rats: evidence for mobile mitochondrial pools", J PERIPH N, 5(1), 2000, pp. 3-10

Abstract

We report for the first time the rearrangement of mitochondrial arrays in developing dorsal root ganglion (DRG) neurons isolated from neonatal rats in culture. Neurons were loaded with the mitochondria-specific fluorescent dye JC-1, and three-dimensional (3D) reconstruction of mitochondrial fluorescence was performed by confocal laser sectioning in fresh neurons and neurons kept in culture up to a week. We found that after 24 hours the mitochondria become reorganised to form clusters in the axonal hillocks. Axonal extension and neuronal network formation coincided with a redistribution of themitochondrial clusters. In the extended axons the mitochondria become spaced along the axonal length; however,they formed clusters in the branch points and growth cones. We conclude that the initial clusters of mitochondria may be storage pools of mobile mitochondria able to be mobilised to provideenergy for axonal transport during neuronal regeneration and neuronal outgrowth. These findings may have relevance to the rate of axonal regenerationand axonal transport in adult DRG neurons, and neuronal polarisation and axonal outgrowth regulation in developing DRG neurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:07:24