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Titolo:
Evaluation of the effects of intramyocardial injection of DNA expressing vascular endothelial growth factor (VEGF) in a myocardial infarction model in the rat - Angiogenesis and angioma formation
Autore:
Schwarz, ER; Speakman, MT; Patterson, M; Hale, SS; Isner, JM; Kedes, LH; Kloner, RA;
Indirizzi:
Good Samaritan Hosp, Inst Heart, Los Angeles, CA 90017 USA Good Samaritan Hosp Los Angeles CA USA 90017 t, Los Angeles, CA 90017 USA Univ So Calif, Div Cardiol, Los Angeles, CA USA Univ So Calif Los AngelesCA USA Calif, Div Cardiol, Los Angeles, CA USA St Elisabeths Med Ctr, Div Cardiovasc Res, Boston, MA USA St Elisabeths Med Ctr Boston MA USA , Div Cardiovasc Res, Boston, MA USA Tufts Univ, Sch Med, Boston, MA 02111 USA Tufts Univ Boston MA USA 02111Tufts Univ, Sch Med, Boston, MA 02111 USA Univ So Calif, Dept Med, Los Angeles, CA USA Univ So Calif Los Angeles CAUSA So Calif, Dept Med, Los Angeles, CA USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA USA Univ So CalifLos Angeles CA USA Biochem & Mol Biol, Los Angeles, CA USA
Titolo Testata:
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
fascicolo: 5, volume: 35, anno: 2000,
pagine: 1323 - 1330
SICI:
0735-1097(200004)35:5<1323:EOTEOI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECT GENE-TRANSFER; PERIPHERAL ARTERY DISEASE; COLLATERAL BLOOD-FLOW; THERAPEUTIC ANGIOGENESIS; ISCHEMIC MYOCARDIUM; TUMOR ANGIOGENESIS; MUSCLE INVIVO; FACTOR FAMILY; HEART INVIVO; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Kloner, RA Good Samaritan Hosp, Inst Heart, 1225 Wilshire Blvd, Los Angeles, CA 90017USA Good Samaritan Hosp 1225 Wilshire Blvd Los Angeles CA USA 90017
Citazione:
E.R. Schwarz et al., "Evaluation of the effects of intramyocardial injection of DNA expressing vascular endothelial growth factor (VEGF) in a myocardial infarction model in the rat - Angiogenesis and angioma formation", J AM COL C, 35(5), 2000, pp. 1323-1330

Abstract

OBJECTIVES The effects of direct intramyocardial injection of the plasmid encoding vascular endothelial growth factor (phVEGF(165)) in the border zone of myocardial infarct tissue in rat hearts were investigated. BACKGROUND Controversy exists concerning the ability of VEGF to induce angiogenesis and enhance coronary flow in the myocardium. METHODS Sprague-Dawley rats received a ligation of the left coronary artery to induce myocardial infarction (MI). At 33.1 +/- 6.5 days, the rats wereinjected with phVEGF(165) at one location and control plasmid at a second location (500 mu g DNA, n = 24) or saline (n = 16). After 33.1 +/- 5.7 days, the hearts were excised for macroscopic and histologic analysis. Regionalblood flow ratios were measured in 18 rats by radioactive microspheres. RESULTS phVEGF(165)-treated sites showed macroscopic angioma- like structures at the injection site while control DNA and saline injection sites did not. By histology, 21/24 phVEGF(165)-treated hearts showed increased focal epicardial blood vessel density and angioma-like formation. Quantitative morphometric evaluation in 20 phVEGF(165)-treated hearts revealed 44.4 +/- 10.5 vascular structures per field in phVEGF(165)-treated hearts versus 21.4 /- 4.7 in control DNA injection sites (p < 0.05). Regional myocardial blood flow ratios between the injection site and noninfarcted area did not demonstrate any difference between phVEGF(165)-treated hearts (0.9 +/- 0.2) andsaline-treated hearts (0.7 +/- 0.1). CONCLUSIONS Injection of DNA for VEGF in the border zone of MI in rat hearts induced angiogenesis. Angioma formation at the injection sites did not appear to contribute to regional myocardial blood how, which may be a limitation of gene therapy for this application. a Am Coil Cardiol 2000;35:1323-30) (C) 2000 by the American College of Cardiology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 14:27:07