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Titolo:
Enhancement of angiogenesis by the implantation of self bone marrow cells in a rat ischemic heart model
Autore:
Kobayashi, T; Hamano, K; Li, TS; Katoh, T; Kobayashi, S; Matsuzaki, M; Esato, K;
Indirizzi:
Yamaguchi Univ, Sch Med, Dept Surg 1, Yamaguchi 7558505, Japan Yamaguchi Univ Yamaguchi Japan 7558505 Surg 1, Yamaguchi 7558505, Japan Yamaguchi Univ, Sch Med, Dept Physiol 1, Yamaguchi 7558505, Japan Yamaguchi Univ Yamaguchi Japan 7558505 ysiol 1, Yamaguchi 7558505, Japan Yamaguchi Univ, Sch Med, Dept Internal Med 2, Yamaguchi 7558505, Japan Yamaguchi Univ Yamaguchi Japan 7558505 l Med 2, Yamaguchi 7558505, Japan
Titolo Testata:
JOURNAL OF SURGICAL RESEARCH
fascicolo: 2, volume: 89, anno: 2000,
pagine: 189 - 195
SICI:
0022-4804(200004)89:2<189:EOABTI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; FIBROBLAST GROWTH-FACTOR; ENDOTHELIAL-CELLS; TRANSPLANTATION; EXPRESSION; CDNA; GENE; CINC;
Keywords:
angiogenesis; cell; ischemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Hamano, K Yamaguchi Univ, Sch Med, Dept Surg 1, 1-1-1 Minamikogushi, Yamaguchi 7558505, Japan Yamaguchi Univ 1-1-1 Minamikogushi Yamaguchi Japan 7558505 apan
Citazione:
T. Kobayashi et al., "Enhancement of angiogenesis by the implantation of self bone marrow cells in a rat ischemic heart model", J SURG RES, 89(2), 2000, pp. 189-195

Abstract

Background. Bone marrow contains various kinds of primitive cells which differentiate into endothelial cells and could secrete several growth factors. Therefore, we attempted to induce therapeutic angiogenesis using self bone marrow cells in a rat model. Materials and methods. Quantitative angiogenesis was examined using a sponge implantation assay that indicated whether the rat bone marrow cells had induced angiogenesis or not. Employing a rat ischemic heart model, bone marrow cells were injected directly into the ischemic area and the number of vessels was examined immunohistochemically using the anti-CD31 monoclonal antibody. The contributed growth factors revealed the levels present in the ischemic myocardium by an enzyme-linked immunosorbent assay and reverse transcription polymerase chain reaction. Results. The sponge implantation assay showed that bone marrow cells induced angiogenesis. Light microscopic analysis of the vessel count positively stained by anti-CD31 in the ischemic area showed that angiogenesis had beeninduced to a significantly greater degree in the group implanted with bonemarrow cells (BMI group) than in the group injected with phosphate-buffered saline (PBS group) 1 week after BMI. Levels of the inflammatory cytokinesinterleukin-1 (IL-1 beta) and cytokine-induced neutrophil chemoattractant (CINC) in the BMI group were significantly elevated compared with those in the PBS group. Conclusions. Self bone marrow cell implantation induced angiogenesis in a rat ischemic heart model as a result of elevation of the levels of IL-1 beta and CINC. Thus, bone marrow implantation could be a novel and simple method to induce therapeutic angiogenesis. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:28:48