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Titolo:
Evidence for the non-involvement of nitric oxide synthase in nitric oxide production by the green alga Scenedesmus obliquus
Autore:
Mallick, N; Mohn, FH; Rai, LC; Soeder, CJ;
Indirizzi:
KFA Julich GmbH, Forschungszentrum, D-52425 Julich, Germany KFA Julich GmbH Julich Germany D-52425 szentrum, D-52425 Julich, Germany Banaras Hindu Univ, Dept Bot, Varanasi 221005, Uttar Pradesh, India Banaras Hindu Univ Varanasi Uttar Pradesh India 221005 tar Pradesh, India
Titolo Testata:
JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 3, volume: 156, anno: 2000,
pagine: 423 - 426
SICI:
0176-1617(200003)156:3<423:EFTNON>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANTS;
Keywords:
Scenedesmus; NOS; N-W-nitro-L-arginine; N-G-nitro-L-arginine-methyl ester; nitric oxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Mallick, N KFA Julich GmbH, Forschungszentrum, ICG-6, D-52425 Julich, Germany KFA Julich GmbH ICG-6 Julich Germany D-52425 5 Julich, Germany
Citazione:
N. Mallick et al., "Evidence for the non-involvement of nitric oxide synthase in nitric oxide production by the green alga Scenedesmus obliquus", J PLANT PHY, 156(3), 2000, pp. 423-426

Abstract

Studies conducted with N-W-nitro-L-arginine (L-NNA) and N-G-nitro-L-arginine-methyl ester (L-NAME), the potent inhibitors of nitric oxide synthase (NOS), did not exhibit any inhibitory effect on NO release by Scenedesmus cells grown either under illumination or in the dark supplemented with glucose. Further, these inhibitors did not interfere with the appearance of 'light-off' peaks, and the NO peaks also commenced following treatment with Cu, DCMU and glutarate in the presence of L-NNA and L-NAME. Complete suppressionof NO production and the elimination of the most common 'light-off' peak were observed in algal culture grown in the medium where L-arginine was substituted for NO3-. This study therefore demonstrates that L-arginine is neither the substrate for NO production by Scenedesmus, as generally found in the case of animals and some plants, nor is the NOS involved in the nitric oxide production process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 09:25:18