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Titolo:
The function of plakophilin 1 in desmosome assembly and actin filament organization
Autore:
Hatzfeld, M; Haffner, C; Schulze, K; Vinzens, U;
Indirizzi:
Univ Halle Wittenberg, Fac Med, Mol Biol Grp, D-06097 Halle, Germany Univ Halle Wittenberg Halle Germany D-06097 Grp, D-06097 Halle, Germany
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 1, volume: 149, anno: 2000,
pagine: 209 - 222
SICI:
0021-9525(20000403)149:1<209:TFOP1I>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE KINASE SUBSTRATE; CADHERIN-PLAKOGLOBIN COMPLEXES; INTERMEDIATE FILAMENTS; PROTEIN INTERACTIONS; EPITHELIAL-CELLS; CLASSICAL CADHERINS; PLAQUE COMPONENTS; MULTIGENE FAMILY; BAND-6 PROTEIN; BINDING-SITES;
Keywords:
keratinocytes; desmoglein; armadillo; cell adhesion; cell motility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Hatzfeld, M Univ Halle Wittenberg, Fac Med, Mol Biol Grp, Magdeburger Str 18, D-06097 Halle, Germany Univ Halle Wittenberg Magdeburger Str 18 Halle Germany D-06097
Citazione:
M. Hatzfeld et al., "The function of plakophilin 1 in desmosome assembly and actin filament organization", J CELL BIOL, 149(1), 2000, pp. 209-222

Abstract

Plakophilin 1, a member of the ar armadillo multigene family, is a proteinwith dual localization in the nucleus and in desmosomes. To elucidate its role in desmosome assembly and regulation, we have analyzed its localization and binding partners in vivo. When overexpressed in HaCaT keratinocytes, plakophilin 1 localized to the nucleus and to desmosomes, and dramatically enhanced the recruitment of desmosomal proteins to the plasma membrane. This effect was mediated by plakophilin 1's head domain, which interacted withdesmoglein 1, desmoplakin, and keratins in the yeast two-hybrid system. Overexpression of the armadillo repeat domain induced a striking dominant negative pheno-type with the formation of filopodia and long cellular protrusions, where plakophilin 1 colocalized with actin filaments. This phenotype was strictly dependent on a conserved motif in the center of the armadillo repeat domain. Our results demonstrate that plakophilin 1 contains two functionally distinct domains: the head domain, which could play a role in organizing the desmosomal plaque in suprabasal cells, and the armadillo repeat domain, which might be involved in regulating the dynamics of the actin cytoskeleton.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:42:32