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Titolo:
G-CSF receptor expression in ovarian cancer
Autore:
Ninci, EB; Brandstetter, T; Meinhold-Heerlein, I; Bettendorf, H; Sellin, D; Bauknecht, T;
Indirizzi:
Univ Freiburg, Dept Obstet & Gynecol, Freiburg, Germany Univ Freiburg Freiburg Germany Dept Obstet & Gynecol, Freiburg, Germany Burnham Inst, J Reed Lab, La Jolla, CA 92037 USA Burnham Inst La Jolla CAUSA 92037 st, J Reed Lab, La Jolla, CA 92037 USA Univ Bonn, Dept Obstet & Gynecol, D-5300 Bonn, Germany Univ Bonn Bonn Germany D-5300 ept Obstet & Gynecol, D-5300 Bonn, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
fascicolo: 1, volume: 10, anno: 2000,
pagine: 19 - 26
SICI:
1048-891X(200001/02)10:1<19:GREIOC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; GRANULOCYTE-COLONY; SIGNAL TRANSDUCER; CELL-LINE; HEMATOPOIETIN RECEPTOR; MOLECULAR-CLONING; IN-VITRO; GROWTH; GP130; PROLIFERATION;
Keywords:
granulocyte colony-stimulating factor (G-CSF); ovarian cancer; receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Ninci, EB Klin Tumorbiol, 5th Floor,Breisacherstr 117, D-79106 Freiburg, Germany Klin Tumorbiol 5th Floor,Breisacherstr 117 Freiburg Germany D-79106
Citazione:
E.B. Ninci et al., "G-CSF receptor expression in ovarian cancer", INT J GYN C, 10(1), 2000, pp. 19-26

Abstract

Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is clinically used to overcome neutropenic periods during chemotherapy. In vitro studies using cell lines as a model system have recently suggested that G-CSFcan promote ovarian cancer growth. The objective of this work is to determine whether tumor cells express G-CSF-receptors (G-CSFR). A set of ovarian tumor biopsies and ovarian cancer cell lines was analyzed by RT-PCR, immunohistochemistry and immunofluorescence. The presence of a 276 bp-amplicon (exon 8-10) obtained by RT-PCR showed that 12 out of 16 ovarian tumor biopsies and two out of four ovarian cancer cell lines expressed G-CSFR-mRNA. G-CSFR-protein was detected in tumor cells of the 12 biopsies that also contained G-CSFR-mRNA. A second 409 bp-amplicon (exon 17) obtained by RT-PCR from the variable C-terminal cytoplasmic region of G-CSFR could be amplified only in four out of 16 biopsies and in none of the ovarian cancer cell lines studied. The results presented here indicate that G-CSFR is frequently expressed in ovarian cancer cells. Moreover, the failure of RT-PCR amplificationof the 409 bp-amplicon in samples that express G-CSFR-mRNA suggests that C-terminal truncated receptor forms are also expressed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 10:41:30