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Titolo:
Extracellular cyclic ADP-ribose increases intracellular free calcium concentration and stimulates proliferation of human hemopoietic progenitors
Autore:
Podesta, M; Zocchi, E; Pitto, A; Usai, C; Franco, L; Bruzzone, S; Guida, L; Bacigalupo, A; Scadden, DT; Walseth, TF; De Flora, A; Daga, A;
Indirizzi:
Univ Genoa, Dept Expt Med, Biochem Sect, I-16132 Genoa, Italy Univ Genoa Genoa Italy I-16132 t Med, Biochem Sect, I-16132 Genoa, Italy S Martino Hosp, Dept Hematol, Genoa, Italy S Martino Hosp Genoa ItalyS Martino Hosp, Dept Hematol, Genoa, Italy Natl Res Council, Inst Cybernet & Biophys, Genoa, Italy Natl Res Council Genoa Italy cil, Inst Cybernet & Biophys, Genoa, Italy Harvard Univ, Sch Med, Dept Expt Hematol, Massachusetts Gen Hosp, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 achusetts Gen Hosp, Boston, MA 02115 USA Univ Minnesota, Dept Pharmacol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 rmacol, Minneapolis, MN 55455 USA Natl Inst Canc Res, IST, Genoa, Italy Natl Inst Canc Res Genoa ItalyNatl Inst Canc Res, IST, Genoa, Italy
Titolo Testata:
FASEB JOURNAL
fascicolo: 5, volume: 14, anno: 2000,
pagine: 680 - 690
SICI:
0892-6638(200004)14:5<680:ECAIIF>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; INOSITOL TRISPHOSPHATE; CYCLASE ACTIVITY; RYANODINE RECEPTORS; ENDOGENOUS LEVELS; MICE LACKING; HUMAN CD38; RADIOIMMUNOASSAY; DIFFERENTIATION; GLYCOPROTEIN;
Keywords:
bone marrow cells; intracellular calcium homeostasis; cytokine-like activity of cyclic ADP-ribose; expansion of hemopoietic progenitors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: De Flora, A Univ Genoa, Dept Expt Med, Biochem Sect, Viale Benedetto XV-1,I-16132 Genoa, Italy Univ Genoa Viale Benedetto XV-1 Genoa Italy I-16132 oa, Italy
Citazione:
M. Podesta et al., "Extracellular cyclic ADP-ribose increases intracellular free calcium concentration and stimulates proliferation of human hemopoietic progenitors", FASEB J, 14(5), 2000, pp. 680-690

Abstract

Cyclic ADP-ribose (cADPR) is a universal second messenger that regulates many calcium-related cellular events by releasing calcium from intracellularstores. Since these events include enhanced cell proliferation and since the bone marrow harbors both ectoenzymes that generate cADPR from NAD(+) (CD38 and BST-1), we investigated the effects of extracellular cADPR on human hemopoietic progenitors (HP). Exposure of HE to 100 mu M cADPR for 24 h induced a significant increase in colony output (P<0.01) and colony size (P<0.003). A horizontal expansion of HP, as demonstrated by a markedly increasedreplating efficiency in semisolid medium (up to 700 times compared to controls), was also observed, indicating that cADPR priming can affect cell growth for multiple generations over several weeks after exposure. Influx of extracellular cADPR into the cells was demonstrated, and a causal relationship between the functional effects and the increase of intracellular free calcium concentration induced by cADPR on HP was established through the use of specific antagonists. Similar effects on HP were produced by nanomolar concentrations of the nonhydrolyzable cADPR analog 3-deaza-cADPR, These datademonstrate that extracellular cADPR behaves as a cytokine enhancing the proliferation of human HP, a finding that may have biomedical applications for the ex vivo expansion of hemopoietic cells.-Podesta, M., Zocchi, E., Pitto, A, Usai, C., Franco, L., Bruzzone, S., Guida, L., Bacigalupo, A., Scadden, D. T., Walseth, T. F., De Flora, A., Daga, A Extracellular cyclic ADP-ribose increases intracellular free calcium concentration and stimulates proliferation of human hemopoietic progenitors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 19:02:27