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Titolo:
A comparison of three commercially available isoelectric focusing units for proteome analysis: The Multiphor, the IPGphor and the Protean IEF cell
Autore:
Choe, LH; Lee, KH;
Indirizzi:
Cornell Univ, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853Cornell Univ, Ithaca, NY 14853 USA
Titolo Testata:
ELECTROPHORESIS
fascicolo: 5, volume: 21, anno: 2000,
pagine: 993 - 1000
SICI:
0173-0835(200003)21:5<993:ACOTCA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMOBILIZED PH GRADIENTS; 2-DIMENSIONAL ELECTROPHORESIS;
Keywords:
isoelectric focusing; immobilized pH gradients; proteome analysis; two-dimensional protein electrophoresis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, KH Cornell Univ, 120 Olin Hall, Ithaca, NY 14853 USA Cornell Univ 120Olin Hall Ithaca NY USA 14853 thaca, NY 14853 USA
Citazione:
L.H. Choe e K.H. Lee, "A comparison of three commercially available isoelectric focusing units for proteome analysis: The Multiphor, the IPGphor and the Protean IEF cell", ELECTROPHOR, 21(5), 2000, pp. 993-1000

Abstract

We tested and compared three different commercially available instruments for isoelectric focusing for proteome analysis by two-dimensional protein electrophoresis. These instruments, the Multiphor, the IPGphor, and the Protean IEF cell, were used with 18 cm immobilized pH gradient strips and run under various conditions. The total number of spots and features was obtained by Melanie software (Bio-Rad Laboratories) and separately by visual inspection. The Multiphor consistently resulted in the highest number of spots detected per gel independent of sample type, immobilized pH gradient (IPG) and method to calculate the number of spots. The Protean IEF cell had the next highest number of spots detected per gel. In the experiments performed, the IPGphor afforded good reproducibility in the total number of Melanie-detected spots from gel to gel while the Protean IEF cell offered better reproducibility in the total number of manually detected spots from gel to gel. Among gels run with the different instruments, differences in the quality of the ammoniacal silver stain were also observed. A measure of quantitative reproducibility suggests that the Protean IEF cell, which was the easiestinstrument to use, performs better than the other instruments, although all three instruments had demonstrated good quantitative reproducibility in the experiments performed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 03:29:34