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Titolo:
Localisation of Xho1 repetitive sequences on autosomes in addition to the W chromosome in chickens and its relevance for sex diagnosis
Autore:
Klein, S; Ellendorff, F;
Indirizzi:
Fed Agr Res Ctr, Inst Anim Sci & Anim Behav Mariensee, D-31535 Neustadt, Germany Fed Agr Res Ctr Neustadt Germany D-31535 nsee, D-31535 Neustadt, Germany
Titolo Testata:
ANIMAL GENETICS
fascicolo: 2, volume: 31, anno: 2000,
pagine: 104 - 109
SICI:
0268-9146(200004)31:2<104:LOXRSO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYOS;
Keywords:
chicken; sex diagnosis; repetitive DNA; FISH; PCR; single cell detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Klein, S Fed Agr Res Ctr, Inst Anim Sci & Anim Behav Mariensee, Holtystr 10, D-31535 Neustadt, Germany Fed Agr Res Ctr Holtystr 10 Neustadt Germany D-31535 dt, Germany
Citazione:
S. Klein e F. Ellendorff, "Localisation of Xho1 repetitive sequences on autosomes in addition to the W chromosome in chickens and its relevance for sex diagnosis", ANIM GENET, 31(2), 2000, pp. 104-109

Abstract

Repetitive sequences of the Xho1 family comprise about 70% of the W chromosome in chickens, are considered to be restricted to this sex chromosome and are used in sex diagnosis. This study investigated the reliability of sexdiagnosis on small cell numbers. Fluorescent in situ hybridisations (FISH)with the Xho1 repeat sequence were performed on metaphases and signals were detected on the W chromosome and on some autosomes. Differences in signalintensity suggested that the number of repeats ranged from several thousand on the W chromosome to only a few on the autosomes. These results confirmthe report from Clinton (1994) of minor male products in sex specific PCR of the Xho1 repeat. We adopted his PCR protocol to analyse small numbers ofcells. FISH on blastomeres and on blood cells from E3 to E11 embryos were compared to PCR results. The FISH procedure as well as the PCR protocol aresuitable for sexing. For single cells FISH was slightly more consistent.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:14:33