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Titolo:
Identification and quantification of flavonoids in human urine samples by column switching liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry
Autore:
Nielsen, SE; Freese, R; Cornett, C; Dragsted, LO;
Indirizzi:
Danish Vet & Food Adm, Inst Food Safety & Toxicol, DK-2860 Soborg, DenmarkDanish Vet & Food Adm Soborg Denmark DK-2860 ol, DK-2860 Soborg, Denmark Univ Helsinki, Div Nutr, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 tr, FIN-00014 Helsinki, Finland Royal Danish Sch Pharm, Inst Pharmaceut & Anal Chem, DK-2100 Copenhagen, Denmark Royal Danish Sch Pharm Copenhagen Denmark DK-2100 00 Copenhagen, Denmark
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 7, volume: 72, anno: 2000,
pagine: 1503 - 1509
SICI:
0003-2700(20000401)72:7<1503:IAQOFI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRAVIOLET ABSORBENCY DETECTION; CORONARY-HEART-DISEASE; QUERCETIN; CANCER; ABSORPTION; VOLUNTEERS; NARINGIN; ASSAY; HPLC; RISK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Nielsen, SE Danish Vet & Food Adm, Inst Food Safety & Toxicol, Morkhoj Bygade 19, DK-2860 Soborg, Denmark Danish Vet & Food Adm Morkhoj Bygade 19 Soborg Denmark DK-2860
Citazione:
S.E. Nielsen et al., "Identification and quantification of flavonoids in human urine samples by column switching liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry", ANALYT CHEM, 72(7), 2000, pp. 1503-1509

Abstract

A rapid and sensitive high-performance liquid chromatographic mass spectrometric (HPLC-MS) method is described for the determination and quantification of 12 dietary flavonoid glycosides and aglycons in human urine samples. Chromatographic separation of the analytes of interest was achieved by column-switching, using the first column (a Zorbax 300SB C-3 column) for samplecleanup and eluting the heart-cut flavonoid fraction onto the second column (a Zorbax SE C-18 column) for separation and detection by ultraviolet andatmospheric pressure chemical ionization MS using single ion monitoring innegative mode. The fragmentor voltage was optimized with regard to maximumabundance of the molecular ion and qualifier ions of the analytes. Calibration graphs were prepared for urine, and good linearity was achieved over adynamic range of 2.5-1000 ng/mL, The inter- and intraassay coefficients ofvariation for the analysis of the 12 different flavonoids in quality control urine samples were 12.3% on average (range 11.0-13.7%, n = 24, reproducibility) and the repeatability of the assay were 5.0% (mean, range 0.1-14.8%, it = 12). A subset of 10 urine samples from a human dietary intervention study with high and low flavonoid content was analyzed, and the results arereported.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 14:00:13