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Titolo:
Deactivation of TSH receptor signaling in filter-cultured pig thyroid epithelial cells
Autore:
Ericson, LE; Nilsson, M;
Indirizzi:
Univ Gothenburg, Inst Anat & Cell Biol, SE-40530 Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden SE-40530 , SE-40530 Gothenburg, Sweden
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 4, volume: 278, anno: 2000,
pagine: E611 - E619
SICI:
0193-1849(200004)278:4<E611:DOTRSI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYROTROPIN RECEPTOR; CYCLIC-AMP; IODIDE TRANSPORT; APICAL MEMBRANE; PLASMA-MEMBRANE; GROWTH-FACTOR; PROTEIN; RAT; DESENSITIZATION; IODINATION;
Keywords:
cell polarity; vectorial ion transport; transepithelial potential difference; transepithelial resistance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Ericson, LE Univ Gothenburg, Inst Anat & Cell Biol, POB 420, SE-40530 Gothenburg, Sweden Univ Gothenburg POB 420 Gothenburg Sweden SE-40530 rg, Sweden
Citazione:
L.E. Ericson e M. Nilsson, "Deactivation of TSH receptor signaling in filter-cultured pig thyroid epithelial cells", AM J P-ENDO, 278(4), 2000, pp. E611-E619

Abstract

Thyrotropin [thyroid-stimulating hormone (TSH)] receptor on-off signaling was studied in polarized monolayers of pig thyrocytes cultured on permeablesupport. Transepithelial resistance (R) and potential difference (PD) wereused as parameters to monitor the effect of altered TSH concentrations on vectorial electrolyte transport. TSH induced rapid but long-lasting changesin R (decrease) and PD (increase) that were cAMP-dependent and related to enhanced transcellular conductance of sodium and chloride. Withdrawal of TSH from cultures prestimulated with TSH (0.1 mU/ml) for 48 h resulted in restitution of R to control level within 30 min. Such deactivation was markedly accelerated by mild trypsinization, which degraded receptor-bound ligand without affecting TSH receptor responsiveness or ion transporting capacity. Small alterations in the TSH concentration (0.01-0.1 mU/ml) were followed almost instantaneously by adjustments of R. In contrast, the reversal of R after acute TSH stimulation (30 min) and subsequent TSH washout was delayedfor several hours independently of cell surface trypsinization. The observations indicate that, during continuous exposure to physiological concentrations, TSH exerts a close minute-to-minute surveillance of thyroid functionand the rate-limiting step of deactivation is the dissociation of ligand from the TSH receptor at the cell surface. TSH-deprived cells briefly exposed to TSH are refractory to rapid deactivation, probably because of altered metabolism downstream of TSH receptor signal transduction.

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Documento generato il 02/07/20 alle ore 20:15:34