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Titolo:
Shotgun sequencing of the human transcriptome with ORF expressed sequence tags
Autore:
Neto, ED; Correa, RG; Verjovski-Almeida, S; Briones, MRS; Nagai, MA; da Silva, W; Zago, MA; Bordin, S; Costa, FF; Goldman, GH; Carvalho, AF; Matsukuma, A; Baia, GS; Simpson, DH; Brunstein, A; de Oliveira, PSL; Bucher, P; Jongeneel, CV; OHare, MJ; Soares, F; Brentani, RR; Reis, LFL; de Souza, SJ; Simpson, AJG;
Indirizzi:
Ludwig Inst Canc Res, BR-01509010 Sao Paulo, Brazil Ludwig Inst Canc Res Sao Paulo Brazil BR-01509010 BC10 Sao Paulo, Brazil Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05599970 Sao Paulo, Brazil Univ Sao Paulo Sao Paulo Brazil BR-05599970 BC05599970 Sao Paulo, Brazil Univ Fed Sao Paulo, Escola Paulista Med, BR-04023062 Sao Paulo, Brazil Univ Fed Sao Paulo Sao Paulo Brazil BR-04023062 BC3062 Sao Paulo, Brazil Univ Sao Paulo, Fac Med, Dept Radiol, BR-01296903 Sao Paulo, Brazil Univ Sao Paulo Sao Paulo Brazil BR-01296903 BC01296903 Sao Paulo, Brazil Fac Med Ribeirao Preto, Dept Clin Med, BR-14049900 Sao Paulo, Brazil Fac Med Ribeirao Preto Sao Paulo Brazil BR-14049900 BC Sao Paulo, Brazil Univ Estadual Campinas, Hemoctr, BR-13089970 Sao Paulo, Brazil Univ Estadual Campinas Sao Paulo Brazil BR-13089970 BC Sao Paulo, Brazil Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14049900 Sao Paulo, Brazil Fac Ciencias Farmaceut Ribeirao Preto Sao Paulo Brazil BR-14049900 BCzil Swiss Inst Bioinformat, CH-1066 Epalinges, Switzerland Swiss Inst Bioinformat Epalinges Switzerland CH-1066 linges, Switzerland Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland Swiss Inst Expt Canc Res Epalinges Switzerland CH-1066 nges, Switzerland Ludwig Inst Canc Res, Off Informat Technol, CH-1066 Epalinges, SwitzerlandLudwig Inst Canc Res Epalinges Switzerland CH-1066 palinges, Switzerland Ludwig Inst Canc Res Univ Catholique Louvaine Bre, London W1P 7LD, EnglandLudwig Inst Canc Res Univ Catholique Louvaine Bre London England W1P 7LD Hosp AC Camargo, BR-01509010 Sao Paulo, Brazil Hosp AC Camargo Sao PauloBrazil BR-01509010 BC1509010 Sao Paulo, Brazil
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 7, volume: 97, anno: 2000,
pagine: 3491 - 3496
SICI:
0027-8424(20000328)97:7<3491:SSOTHT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PROSTATE; GENERATION; CANCER; DISCOVERY; GENES; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Simpson, AJG Ludwig Inst Canc Res, BR-01509010 Sao Paulo, Brazil Ludwig Inst Canc Res Sao Paulo Brazil BR-01509010 BC Brazil
Citazione:
E.D. Neto et al., "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags", P NAS US, 97(7), 2000, pp. 3491-3496

Abstract

Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription-PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. systematic, high-throughput sequencing of such products would result in an expressed sequence tag (EST) database consisting of central, generally coding regions of expressed genes. Such a database would add significant value to existing public EST databases, which consist mostly of sequences derived from the extremities of cDNAs, and facilitate the construction of contigs of transcript sequences. We tested our predictions, creating a database of 10,000 sequences from human breast tumors. The data confirmed thecentral distribution of the sequences, the significant normalization of the sequence population, the frequent extension of contigs composed of existing human ESTs, and the identification of a series of potentially important homologues of known genes. This approach should make a significant contribution to the early identification of important human genes, the deciphering of the draft human genome sequence currently being compiled, and the shotgun sequencing of the human transcriptome.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 00:03:55