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Titolo:
Induction of erythroid differentiation by inhibition of Ras/ERK pathway ina Friend murine leukemia cell line
Autore:
Matsuzaki, T; Aisaki, K; Yamamura, Y; Noda, M; Ikawa, Y;
Indirizzi:
Tokyo Med & Dent Univ, Dept Retroviral Regulat, Div Med Res, Bunkyo Ku, Tokyo 1138519, Japan Tokyo Med & Dent Univ Tokyo Japan 1138519 unkyo Ku, Tokyo 1138519, Japan Japan Sci & Technol Corp, CREST, Honcho, Saitama 3320012, Japan Japan Sci & Technol Corp Honcho Saitama Japan 3320012 tama 3320012, Japan Kyoto Univ, Grad Sch Med, Dept Mol Oncol, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Mol Oncol, Sakyo Ku, Kyoto 6068501, Japan
Titolo Testata:
ONCOGENE
fascicolo: 12, volume: 19, anno: 2000,
pagine: 1500 - 1508
SICI:
0950-9232(20000316)19:12<1500:IOEDBI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE ERYTHROLEUKEMIA-CELLS; SIGNAL-TRANSDUCTION PATHWAY; PROTEIN-KINASE CASCADE; P38 MAP KINASE; ERYTHROPOIETIN RECEPTOR; TYROSINE PHOSPHORYLATION; PHOSPHATIDYLINOSITOL 3-KINASE; GENE-EXPRESSION; ACTIVATION; JAK2;
Keywords:
Friend leukemia cell; EPO-induced differentiation; Ras/ERK pathway;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Ikawa, Y Tokyo Med & Dent Univ, Dept Retroviral Regulat, Div Med Res, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138519, Japan Tokyo Med & Dent Univ 1-5-45 Yushima Tokyo Japan 1138519 , Japan
Citazione:
T. Matsuzaki et al., "Induction of erythroid differentiation by inhibition of Ras/ERK pathway ina Friend murine leukemia cell line", ONCOGENE, 19(12), 2000, pp. 1500-1508

Abstract

The role of Ras and MAP kinases (MAPKs) in the regulation of erythroid differentiation was studied using a cell line (SKT6) derived from Friend virus(Anemic strain)-induced murine erythroleukemia. This cell line undergoes differentiation in vitro in response to erythropoietin (EPO) or other chemical inducers such as dimethylsulfoxide (DMSO), When a constitutively active ras mutant (ras12V) was expressed in SKT6 cells, EPO-induced differentiation was inhibited. Conversely, a dominant negative I au mutant (ras17N) induced differentiation even in the absence of EPO, suggesting that the basal Ras activity is essential for the maintenance of the undifferentiated phenotype and proliferative potential in this cell line. Rapid inactivation of ERKwas observed after expression of ras17N. Slow but significant inactivationof ERR was also observed during EPO-induced differentiation. Furthermore, overexpression of a constitutively active mutant of ERK-activating kinase (MAPKK) was found to suppress erythroid differentiation, while pharmacological inhibition of MAPKK induced differentiation. These findings suggest thatdown-regulation of Ras/ERK signaling pathway may be an essential event in EPO-induced erythroid differentiation in this system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:33:09