Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The role of nuclear cap binding protein Cbc1p of yeast in mRNA terminationand degradation
Autore:
Das, B; Guo, ZJ; Russo, P; Chartrand, P; Sherman, F;
Indirizzi:
Univ Rochester, Sch Med, Dept Biochem & Biophys, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 & Biophys, Rochester, NY 14642 USA Yeshiva Univ Albert Einstein Coll Med, Inst Mol Med, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA Cornell Univ IthacaNY USA 14853 Dept Plant Pathol, Ithaca, NY 14853 USA
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 8, volume: 20, anno: 2000,
pagine: 2827 - 2838
SICI:
0270-7306(200004)20:8<2827:TRONCB>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA DECAY; NONSENSE-MEDIATED DECAY; SACCHAROMYCES-CEREVISIAE; POLYADENYLATION SIGNAL; GLYCOLYTIC GENES; 3'-END FORMATION; POLY(A) TAIL; TRANSLATION; SEQUENCE; COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
93
Recensione:
Indirizzi per estratti:
Indirizzo: Sherman, F Univ Rochester, Sch Med, Dept Biochem & Biophys, Box 712, Rochester, NY 14642 USA Univ Rochester Box 712 Rochester NY USA 14642 ter, NY 14642 USA
Citazione:
B. Das et al., "The role of nuclear cap binding protein Cbc1p of yeast in mRNA terminationand degradation", MOL CELL B, 20(8), 2000, pp. 2827-2838

Abstract

The cyc1-512 mutation in Saccharomyces cerevisiae causes a 90% reduction in the level of iso-1-cytochrome c because of the lack of a proper 3'-end-forming signal, resulting in low levels of eight aberrantly long cyc1-512 mRNAs which differ in length at their 3' termini, cyc1-512 can be suppressed by deletion of either of the nonessential genes CBC1 and CBC2, which encode the CBP80 and CBP20 subunits of the nuclear cap binding complex, respectively, or by deletion of the nonessential gene UPF1, which encodes a major component of the mRNA surveillance complex. The upf1-Delta deletion suppressedthe cyc1-512 defect by diminishing degradation of the longer subset of cyc1-512 mRNAs, suggesting that downstream elements or structures occurred in the extended 3' region, similar to the downstream elements exposed by transcripts bearing premature nonsense mutations. On the other hand, suppressionof cyc1-512 defects by cbc1-Delta occurred by two different mechanisms, The levels of the shorter cyc1-512 transcripts were enhanced in the cbc1-Delta mutants by promoting 3'-end formation at otherwise-weak sites, whereas the levels of the longer cyc1-512 transcripts, as well as of all mRNAs, were slightly enhanced by diminishing degradation. Furthermore, cbc1-Delta greatly suppressed the degradation of mRNAs and other phenotypes of a rat7-1 strain which is defective in mRNA export. We suggest that Cbc1p defines a novel degradation pathway that acts on mRNAs partially retained in nuclei.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 12:26:17