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Titolo:
BMP2 is required for early heart development during a distinct time period
Autore:
Schlange, T; Andree, B; Arnold, HH; Brand, T;
Indirizzi:
Tech Univ Braunschweig, Inst Biochem & Biotechnol, Dept Cell & Mol Biol, D-38106 Braunschweig, Germany Tech Univ Braunschweig Braunschweig Germany D-38106 raunschweig, Germany
Titolo Testata:
MECHANISMS OF DEVELOPMENT
fascicolo: 1-2, volume: 91, anno: 2000,
pagine: 259 - 270
SICI:
0925-4773(20000301)91:1-2<259:BIRFEH>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE MORPHOGENETIC PROTEINS; CARDIAC MYOCYTE DIFFERENTIATION; ANTERIOR ENDODERM; PRIMITIVE-STREAK; CARDIOVASCULAR-SYSTEM; PRECARDIAC MESODERM; ECTOPIC EXPRESSION; DROSOPHILA EMBRYO; NEURAL INDUCTION; CHICKEN EMBRYOS;
Keywords:
heart development; Noggin; BMP2; NKX2.5; GATA genes; chicken embryo; gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Brand, T Tech Univ Braunschweig, Inst Biochem & Biotechnol, Dept Cell & Mol Biol, Spielmannstr 7, D-38106 Braunschweig, Germany Tech Univ Braunschweig Spielmannstr 7 Braunschweig Germany D-38106
Citazione:
T. Schlange et al., "BMP2 is required for early heart development during a distinct time period", MECH DEVEL, 91(1-2), 2000, pp. 259-270

Abstract

BMP2, like its Drosophila homologue dpp, is an important signaling molecule for specification of cardiogenic mesoderm in vertebrates. Here, we analyzed the time-course of BMP2-requirement for early heart formation in whole chick embryos and in explants of antero-lateral plate mesoderm. Addition of Noggin to explants isolated at stage 4 and cultured for 24 h resulted in loss of NKX2.5, GATA4, eHAND, Mef2A and vMHC expression. At stages 5-8 the individual genes showed differential sensitivity to Noggin addition. While expression of eHAND, NKX2.5 and Mef2A was clearly reduced by Noggin vMHC was only marginally affected. In contrast, GATA4 expression was enhanced after Noggin treatment. The developmental period during which cardiac mesoderm required the presence of BMP signaling in vivo was assessed by implantation of Noggin expressing cells into stage 4-8 embryos which were then cultured until stage 10-11. Complete loss of NKX2.5 and eHAND expression was observedin embryos implanted at stages 4-6, and expression was still suppressed instages 7 and 8 implanted embryos. GATA4 expression was also blocked by Noggin at stage 4, however increased at stages 5, 6 and 7. Explants of centralmesendoderm that normally do not form heart tissue were employed to study the time-course of BMP2-induced cardiac gene expression. The induction of cardiac lineage markers in central mesendoderm of stage 5 embryos was distinct for different genes. While GATA4, -5, -6 and MEF2A were induced to maximal levels within 6 h after BMP2 addition, eHAND and dHAND required 12 h to reach maximum levels of expression. NKX2.5 was induced by 6 h and accumulated over 48 h. vMHC and titin were induced at significant levels only after 48 h of BMP2 addition. These results indicate that cardiac marker genes display distinct expression kinetics after BMP2 addition and differential response to Noggin treatment suggesting complex regulation of myocardial gene expression in the early tubular heart. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 21:51:28