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Titolo:
Reduced H-1-NMR visibility of creatine in isolated rat hearts
Autore:
Schneider, J; Fekete, E; Weisser, A; Neubauer, S; von Kienlin, M;
Indirizzi:
Univ Wurzburg, Dept Biophys, Wurzburg, Germany Univ Wurzburg Wurzburg Germany urzburg, Dept Biophys, Wurzburg, Germany Univ Wurzburg, Dept Med, Wurzburg, Germany Univ Wurzburg Wurzburg Germany iv Wurzburg, Dept Med, Wurzburg, Germany
Titolo Testata:
MAGNETIC RESONANCE IN MEDICINE
fascicolo: 4, volume: 43, anno: 2000,
pagine: 497 - 502
SICI:
0740-3194(200004)43:4<497:RHVOCI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTON NMR-SPECTROSCOPY; ISCHEMIC INSULT; LACTATE; METABOLISM; DEPLETION; LIPIDS; BRAIN;
Keywords:
H-1-MRS; creatine; absolute quantification; visibility; rat heart;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: von Kienlin, M F Hoffman La Roche Pharma, PRPCS, Bldg 68-05B, CH-4070 Basel, Switzerland F Hoffman La Roche Pharma Bldg 68-05B Basel Switzerland CH-4070
Citazione:
J. Schneider et al., "Reduced H-1-NMR visibility of creatine in isolated rat hearts", MAGN RES M, 43(4), 2000, pp. 497-502

Abstract

The aim of this study was to measure the concentration of creatine in Langendorff perfused rat hearts, both by quantitative H-1-MRS and by high-pressure liquid chromatography (HPLC). First, the relaxation times and other parameters affecting absolute quantification by MRS were determined. At 11.75 T, the relaxation times of myocardial creatine were T-1 = 1.1 +/- 0.29 sec (mean +/- SD, n = 5) and T-2 = 56.4 +/- 6.2 ms (n = 9), In phantom experiments the MRS measurements gave accurate values for the known relative concentrations of the detected substances. In glucose-perfused rat hearts, the creatine concentration measured by HPLC was 14.2 +/- 1.9 mmol/kg wet weight (n = 8), in good agreement with literature values. The H-1-MRS measurements,however, resulted in creatine concentrations of only approximately 60% of this value. The application of CHESS-pulses for water suppression led to a further 30% reduction of the creatine MRS signal. These results indicate a reduced H-1-NMR visibility of creatine in the myocardium, which suggests a compartmentation of myocardial creatine into various pools. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:45:30