Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Adenosine A2 receptor stimulation protects the predamaged liver from cold preservation through activation of cyclic adenosine monophosphate-protein kinase A pathway
Autore:
Minor, T; Akbar, S; Yamamoto, Y;
Indirizzi:
Univ Bonn, Surg Clin, Div Surg Res, D-53127 Bonn, Germany Univ Bonn BonnGermany D-53127 Clin, Div Surg Res, D-53127 Bonn, Germany Kyoto Univ, Grad Sch Med, Dept Surg Gastroenterol, Kyoto, Japan Kyoto Univ Kyoto Japan d Sch Med, Dept Surg Gastroenterol, Kyoto, Japan
Titolo Testata:
LIVER TRANSPLANTATION
fascicolo: 2, volume: 6, anno: 2000,
pagine: 196 - 200
SICI:
1527-6465(200003)6:2<196:AARSPT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEART-BEATING DONORS; ISCHEMIA-REPERFUSION; GASEOUS OXYGEN; RAT-LIVER; TRANSPLANTATION; INJURY; ENHANCEMENT; RESTORATION; ALLOGRAFTS; SIGNAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Minor, T Univ Bonn, Surg Clin, Div Surg Res, D-53127 Bonn, Germany Univ Bonn Bonn Germany D-53127 Surg Res, D-53127 Bonn, Germany
Citazione:
T. Minor et al., "Adenosine A2 receptor stimulation protects the predamaged liver from cold preservation through activation of cyclic adenosine monophosphate-protein kinase A pathway", LIVER TRANS, 6(2), 2000, pp. 196-200

Abstract

The shortage of organ donors has led to reconsideration for the use of non-heart-beating donors (NHBDs), However, graft injury caused by warm ischemia in livers From NHBDs strongly affects posttransplantation outcome, The aim of the present study is to investigate the role of adenosine A2 receptor with regard to hepatic viability after cold preservation of NHBD livers. Cardiac arrest was induced in Wistar rats by phrenotomy of the anesthetized nonheparinized animal. After 60 minutes, the livers were excised and flushedwith 60 mi, of histidine-tryptophan-ketoglutarate (HTK) and stored submerged in HTK at 4 degrees C for 24. hours. Reperfusion was performed in vitro after all livers were incubated at 22 degrees C in saline solution to account for the period of slow rewarming during surgical implantation in vivo. Addition of the selective A2-receptor agonist (CGS 21680; 30 mu g/ 100 mL) to the preservation solution resulted in a significant reduction to one quarter of the parenchymal enzyme release of alanine aminotransferase or lactate dehydrogenase on reperfusion and promoted a 2-fold increase in hepatic bile production. This salutory effect was accompanied by a significant increase (40%) in the activity ratio of protein kinase A (PKA) in the liver tissue and could be abrogated in large part by the PKA inhibitor, Rp-cAMPs, Stimulation of the adenosine A2 receptor during harvest and storage of the graft improves maintenance of tissue integrity in liver grafts, A major part ofthis effect, which may represent a promising approach for the use of NHBD grafts, seems to be mediated through activation of PKA Copyright (C) 2000 by the American Association for the Study of Liver Diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 06:56:32