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Titolo:
In vitro cleavage by asbestos fibers of the fifth component of human complement through free-radical generation and kallikrein activation
Autore:
Governa, M; Amati, M; Valentino, M; Visona, I; Fubini, B; Botta, GC; Volpe, AR; Carmignani, M;
Indirizzi:
Univ Ancona, Inst Occupat Hlth, Ancona, Italy Univ Ancona Ancona ItalyUniv Ancona, Inst Occupat Hlth, Ancona, Italy Univ Turin, Dept Inorgan Chem Chem Phys & Chem Mat, Turin, Italy Univ Turin Turin Italy Inorgan Chem Chem Phys & Chem Mat, Turin, Italy Univ Turin, Dept Traumatol Orthopaed & Occupat Med, Turin, Italy Univ Turin Turin Italy Traumatol Orthopaed & Occupat Med, Turin, Italy Catholic Univ Sacred Heart, CNR, Receptor Chem Ctr, I-00168 Rome, Italy Catholic Univ Sacred Heart Rome Italy I-00168 m Ctr, I-00168 Rome, Italy Univ Aquila, Dept Basic & Appl Biol, Pharmacol Sect, I-67100 Laquila, Italy Univ Aquila Laquila Italy I-67100 Pharmacol Sect, I-67100 Laquila, Italy
Titolo Testata:
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
fascicolo: 7, volume: 59, anno: 2000,
pagine: 539 - 552
SICI:
1528-7394(20000414)59:7<539:IVCBAF>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHIONINE OXIDIZING-AGENTS; 5TH COMPONENT; CHEMOTACTIC FACTORS; IRON; C5; DESENSITIZATION; ANAPHYLATOXINS; NEUTROPHILS; REACTIVITY; CLEARANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Governa, M Univ Torrette Ancona, Ist Med Lavoro, Polo Sci Torrette, I-60020 Torrette Di Ancona, AN, Italy Univ Torrette Ancona Polo Sci Torrette Torrette Di Ancona AN Italy I-60020
Citazione:
M. Governa et al., "In vitro cleavage by asbestos fibers of the fifth component of human complement through free-radical generation and kallikrein activation", J TOX E H A, 59(7), 2000, pp. 539-552

Abstract

Chrysotile and crocidolite fibers incubated in normal human plasma (NHP) generated from the C-5 component of complement C-5a-type fragments that stimulated polymorphonuclear leukocyte (PMN) chemotaxis. Absorption of NHP withantiserum against C-5a totally abolished neutrophil chemotactic activity. Asbestos fibers also produced C-5a small peptides in the presence of ethylene glycol bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA) but not ethylene diamine tetraacetic acid (EDTA). Activation of C-5 was significantly inhibited when asbestos fibers were pretreated with iron chelators such as sodium dithionite (DTN), deferoxamine (DFX), or ascorbate (AA). Concentration-related inhibition of C-5 activation was also observed when asbestos fibers were added concurrently to plasma in the presence of DFX, 1,3-dimethyl-2-thiourea (DMTU), a strong hydroxyl scavenger, or aprotinin (APR), a specific protease inhibitor. Further, chrysotile and crocidolite significantly increased plasma kallikrein activity. Data demonstrate that asbestos-induced C-5 activation plays a role in inflammatory reactions characteristic of asbestosis through mechanisms involving iron ions, hydroxyl radicals, and oxidized C-5-like fragments. The ferrous ions present at the asbestos fiber surface trigger this activation and catalyze, via Fenton reaction, theproduction of hydroxyl radicals, which in turn convert native C-5 to an oxidized C-5-like form. This product is then cleaved by kallikrein, activatedby the same asbestos fibers, yielding an oxidized C-5a with the same functional properties as C-5a.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:34:37