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Titolo:
Post-coital sperm recovery and cryopreservation in the Sumatran rhinoceros(Dicerorhinus sumatrensis) and application to gamete rescue in the Africanblack rhinoceros (Diceros bicornis)
Autore:
OBrien, JK; Roth, TL;
Indirizzi:
Cincinnati Zoo & Bot Garden, Ctr Res Endangered Wildlife, Cincinnati, OH 45220 USA Cincinnati Zoo & Bot Garden Cincinnati OH USA 45220 cinnati, OH 45220 USA Univ Cincinnati, Coll Med, Dept Obstet & Gynecol, Cincinnati, OH 45220 USAUniv Cincinnati Cincinnati OH USA 45220 Gynecol, Cincinnati, OH 45220 USA
Titolo Testata:
JOURNAL OF REPRODUCTION AND FERTILITY
fascicolo: 2, volume: 118, anno: 2000,
pagine: 263 - 271
SICI:
0022-4251(200003)118:2<263:PSRACI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CERATOTHERIUM-SIMUM; CALLITHRIX-JACCHUS; SPERMATOZOA; SEMEN; FERTILIZATION; INSEMINATION; CAPACITATION; COLLECTION; VIABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Roth, TL Cincinnati Zoo & Bot Garden, Ctr Res Endangered Wildlife, Cincinnati, OH 45220 USA Cincinnati Zoo & Bot Garden Cincinnati OH USA 45220 OH 45220 USA
Citazione:
J.K. O'Brien e T.L. Roth, "Post-coital sperm recovery and cryopreservation in the Sumatran rhinoceros(Dicerorhinus sumatrensis) and application to gamete rescue in the Africanblack rhinoceros (Diceros bicornis)", J REPR FERT, 118(2), 2000, pp. 263-271

Abstract

Sumatran rhinoceros (Dicerorhinus sumatrensis) sperm samples were collected from a postcopulatory female and characterized to determine their potential for sperm preservation and future use in artificial insemination. Five samples of acceptable quality from one male were used to compare the effect of two cryoprotectants (glycerol and dimethyl sulfoxide (DMSO)) and two post-thaw protocols (untreated and glass wool column) on sperm quality. The percentage of motile spermatozoa, sperm motility index (0-100) and sperm morphology were evaluated subjectively, and viability and acrosomal status wereassessed using fluorescent markers. Evaluations of frozen-thawed spermatozoa were performed over a 6h incubation interval. Post-coital semen samples n = 5; 104.0 +/- 9.1 ml; 2.5 +/- 0.8 x 10(9) total spermatozoa; mean +/- SEM) exhibited a sperm motility index of 56.7 +/- 3.3, and contained 40.2 +/-6.3%, 72.0 +/- 3.2% and 79.8 +/- 6.5% normal, viable and acrosome-intact spermatozoa, respectively. Glycerol and DMSO were equally effective as cryoprotectants and, regardless of post-thaw protocol, samples retained greater than 80% of all pre-freeze characteristic values. Processing semen samples through glass wool yielded higher quality samples, but only half the total number of motile spermatozoa compared with untreated samples. High values for pre-freeze sperm characteristics were also maintained after cryopreservation of epididymal spermatozoa from one black rhinoceros (Diceros bicornis)using the same protocol. In summary, Sumatran rhinoceros spermatozoa of moderate quality can be collected from post-copulatory females. Rhinoceros sperm samples show only slight reductions in quality after cryopreservation and thawing and have potential for use in artificial insemination.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:09:45