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Titolo:
Isolation of morphologically and functionally intact gastric mucosal microvessels rapid communication
Autore:
Tarnawski, A; Arakawa, T; Sekhon, S; Ichikawa, Y; Szabo, I; Sarfeh, IJ;
Indirizzi:
Vet Adm Med Ctr, Dept Med, Long Beach, CA 90822 USA Vet Adm Med Ctr Long Beach CA USA 90822 ept Med, Long Beach, CA 90822 USA Vet Adm Med Ctr, Dept Surg, Long Beach, CA 90822 USA Vet Adm Med Ctr LongBeach CA USA 90822 pt Surg, Long Beach, CA 90822 USA Univ Calif Irvine, Irvine, CA USA Univ Calif Irvine Irvine CA USAUniv Calif Irvine, Irvine, CA USA Osaka City Univ, Dept Internal Med 3, Osaka, Japan Osaka City Univ OsakaJapan ity Univ, Dept Internal Med 3, Osaka, Japan
Titolo Testata:
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
fascicolo: 1, volume: 51, anno: 2000,
pagine: 167 - 175
SICI:
0867-5910(200003)51:1<167:IOMAFI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROVASCULAR ENDOTHELIAL-CELLS; INFLAMMATORY BOWEL-DISEASE; LONG-TERM CULTURE; ETHANOL; PERMEABILITY; INJURY; MODEL;
Keywords:
gastric microvessels; isolation; endothelial cells; prostacyclin; ultrastructure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Tarnawski, A DVA Med Ctr, Gastroenterol Sect, 5901 E 7th St, Long Beach, CA 90822 USA DVA Med Ctr 5901 E 7th St Long Beach CA USA 90822 A 90822 USA
Citazione:
A. Tarnawski et al., "Isolation of morphologically and functionally intact gastric mucosal microvessels rapid communication", J PHYSL PH, 51(1), 2000, pp. 167-175

Abstract

Gastric mucosal microvessels were isolated after arterial perfusion of therat stomach with magnetized iron oxide suspension. After homogenization ofscrapped gastric mucosa, microvessels were initially separated with a highpower magnet and further separated and purified by using a nylon sieve. Aliquots of purified microvessels were assessed for viability, histologic appearance, ultrastructure and generation of prostacyclin. Microvessels were plated on Matrigel and cultured in DMEM with high glucose and 10% FBS for 1,3 or 5 days. After 1, 3 and 5 days of culturing, endothelial viability wasassessed with Fast green exclusion, and the basal and stimulated (with calcium ionophore) generation of prostacyclin was determined by assaying aliquots of the incubating medium for 6-keto PGF(1 alpha). At 1 and 3 hrs after isolation, microvessels demonstrated intact morphologic structures as reflected by transmission EM and 92+/-4% of viable endothelial cells. The microvessels plated on Matrigel maintained good viability for at least 5 days andgenerated prostacyclin at the baseline and following ionophore stimulation. These data demonstrate that isolated microvessels cultured under optimal conditions are fully viable and functional.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:01:23