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Titolo:
Dose-and-time dependent effect of 2,3,7,8-tetrachlorodibenzo-P-dioxin (TCDD) on progesterone secretion by porcine luteal cells cultured in vitro
Autore:
Gregoraszczuk, EL; Wojtowicz, AK; Zabielny, E; Grochowalski, A;
Indirizzi:
Jagiellonian Univ, Inst Zool, Dept Anim Physiol, PL-30060 Krakow, Poland Jagiellonian Univ Krakow Poland PL-30060 hysiol, PL-30060 Krakow, Poland Cracow Univ Technol, Dept Chem, Krakow, Poland Cracow Univ Technol Krakow Poland iv Technol, Dept Chem, Krakow, Poland
Titolo Testata:
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
fascicolo: 1, volume: 51, anno: 2000,
pagine: 127 - 135
SICI:
0867-5910(200003)51:1<127:DDEO2(>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUTEINIZED GRANULOSA-CELLS; IN-UTERO;
Keywords:
TCDD; luteal cells; progesterone secretion; in vitro;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Gregoraszczuk, EL Jagiellonian Univ, Inst Zool, Dept Anim Physiol, Ingardena 6, PL-30060 Krakow, Poland Jagiellonian Univ Ingardena 6 Krakow Poland PL-30060 nd
Citazione:
E.L. Gregoraszczuk et al., "Dose-and-time dependent effect of 2,3,7,8-tetrachlorodibenzo-P-dioxin (TCDD) on progesterone secretion by porcine luteal cells cultured in vitro", J PHYSL PH, 51(1), 2000, pp. 127-135

Abstract

In the current study, to characterize TCDD action during luteal phase of the ovarian cycle, the direct effect of TCDD was investigated in vitro usinga system of monolayer cell culture. Luteal cells isolated from mid-developing corpora lutea were cultured withfour different doses of TCDD (0.1, 1.0, 10.0 and 100 nM). The dose of 0.1nM TCDD had no effect on progesterone (P4) secretion by luteal cells while the doses of 10nM and 100nM in the same, statistically significant manner decreased P4 secretion (p<0.05). The inhibitory effect of TCDD was dependent not only on doses by also on experimental conditions. In cells treated every day for 72 hrs of culture with 0.1nM TCDD, P4 secretion was 71% of basal secretion. 100nM TCDD added only at the beginning of the culture and nor repeated when medium was changed every 24 hrs decreased P4 secretion to 81.8%of basal secretion. The most inhibitory effect was observed in experimentsin which 100nM TCDD was added at the beginning of the culture and medium was not changed for 72 hrs. Secretion of P4 was only 33.9% of that by control cultures. In order to show the time-dependent response to TCDD in terms of P4 secretion, luteal cells were cultured for 24,48, 72 hrs with 0.1 and 100nM TCDD. 85%, 75% and 72% of basal progesterone secretion was noted after 24, 48 and72h respectively in 0.1nM TCDD-treated cells. In 100nM TCDD treated cells the decrease of progesterone secretion was 57%, 67% and 82% of basal secretion after 24, 48 and 72 hrs of culture. These experiments suggest that TCDD by suppressing progesterone secretion by corpora lutea can cause adverse reproductive effects such as early pregnancy failure. Endocrine disrupters that interfere with progesterone production can act as abortifacients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 20:55:29