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Titolo:
RGS7 complex formation and colocalization with the G beta 5 subunit in theadult rat brain and influence on G beta 5 gamma 2-mediated PLC beta signaling
Autore:
Liang, JJ; Chen, HHD; Jones, PG; Khawaja, XZ;
Indirizzi:
Wyeth Ayerst Res, Dept Neurosci, Princeton, NJ 08543 USA Wyeth Ayerst ResPrinceton NJ USA 08543 Neurosci, Princeton, NJ 08543 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 1, volume: 60, anno: 2000,
pagine: 58 - 64
SICI:
0360-4012(20000401)60:1<58:RCFACW>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTPASE-ACTIVATING PROTEINS; HETEROTRIMERIC G-PROTEINS; GAMMA-SUBUNIT; PHOSPHOLIPASE-C; K+ CHANNELS; EXPRESSION; RECEPTORS; BINDING; RETINA; KINASE;
Keywords:
regulator of G protein signaling 7 protein; G protein beta 5 subunit; brain localization; confocal microscopy; phospholipase C beta activity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Khawaja, XZ Wyeth Ayerst Res, Dept Neurosci, CN 8000, Princeton, NJ 08543 USA Wyeth Ayerst Res CN 8000 Princeton NJ USA 08543 , NJ 08543 USA
Citazione:
J.J. Liang et al., "RGS7 complex formation and colocalization with the G beta 5 subunit in theadult rat brain and influence on G beta 5 gamma 2-mediated PLC beta signaling", J NEUROSC R, 60(1), 2000, pp. 58-64

Abstract

This study describes the colocalized distribution and dimeric complex formation between RGS7, a GTPase-activating protein for several heterotrimeric Ga protein families, and the G beta 5 subunit in the adult rat brain. Confocal dual immunofluorescence labeling studies indicated a broad regional specificity in the cellular coexpression between RGS7 and G beta 5 within the cerebral cortical layers I and V-VI, hippocampal formation, caudate-putamen, medial habenula, most thalamic nuclei, and cerebellar molecular and granular layers. In all instances, G beta I-beta 4 immunoreactivities exhibited no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks. Coimmunoprecipitation studies confirmed the selective protein-protein interaction between RGS7 and G beta 5within brain regions that displayed immunohistochemical colocalization. The influence of RGS7 to modulate G beta 5 gamma 2-mediated phosphatidyl inositol (PI) production was examined in COS-7-cotransfected cells. In the presence of G beta 5 gamma 2 only, intracellular PI accumulation was increased by 25% above basal levels; addition of RGS7 produced no significant alteration in G beta 5 gamma 2-mediated PI accumulation. A similar trend was exhibited when full-length RGS7 was substituted with an RGS7 construct lacking the G beta 5-interacting region (G protein gamma-like domain; GGL domain) orwith RGS4. In conclusion, RGS7/G beta 5 dimers occurred within most brain regions in which both proteins were cellularly coexpressed. However, an influence of RGS7 on G beta 5 gamma 2-mediated PLC beta signaling activity wasnot apparent, athough this was in COS-7 cell transfection studies. J. Neurosci. Res. 60:58-64, 2000. (C) 2000 Wiley-Liss, Inc.

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Documento generato il 22/01/21 alle ore 04:01:47