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Titolo:
Expression of mitochondrial protein-coding genes in Tetrahymena pyriformis
Autore:
Edqvist, J; Burger, G; Gray, MW;
Indirizzi:
Dalhousie Univ, Dept Biochem, Canadian Inst Adv Res, Program Evolutionary Biol, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7iol, Halifax, NS B3H 4H7, Canada Univ Montreal, Dept Biochim, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 im, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 2, volume: 297, anno: 2000,
pagine: 381 - 393
SICI:
0022-2836(20000324)297:2<381:EOMPGI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; CAPPED INVITRO; RIBOSOMAL-RNA; DNA; SEQUENCE; GENOME; SUBUNIT; REPLICATION; IDENTIFICATION; PARAMECIUM;
Keywords:
mtDNA; ciliates; transcript mapping; Northern analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Gray, MW Dalhousie Univ, Dept Biochem, Canadian Inst Adv Res, Program Evolutionary Biol, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7 fax, NS B3H 4H7, Canada
Citazione:
J. Edqvist et al., "Expression of mitochondrial protein-coding genes in Tetrahymena pyriformis", J MOL BIOL, 297(2), 2000, pp. 381-393

Abstract

In the ciliate protozoon, Tetrahymena pyriformis, mitochondrial protein-coding genes are highly divergent in sequence, and in a number of cases they lack AUG initiation codons. We asked whether RNA editing might be acting togenerate protein sequences that are more conventional than those inferred from the corresponding gene sequences, and/or to create standard AUG initiation codons where these are absent. However, comparison of genomic and cDNAsequences (the latter generated by reverse transcriptase sequencing of T. pyriformis mitochondrial mRNAs) yielded no evidence of mitochondrial RNA editing in this organism. To delineate the 5' ends of mitochondrial protein-coding transcripts, primer extension experiments were conducted. In all cases, 5' termini were found to map within a few nucleotides of potential initiation codons, indicating that T. pyriformis mitochondrial mRNAs have littleor no 5' untranslated leader sequence. The pattern of strong primer extension stops suggested that both standard (AUG) and non-standard (AUU, AUA, GUG, UUG) initiation codons are utilized by the Tetrahymena mitochondrial translation system. We also investigated expression of the nad1 gene, which inboth T. pyriformis and Paramecium aurelia is split into two portions that are encoded by and transcribed from different DNA strands. Northern hybridization analysis showed that the corresponding transcripts are not trans-spliced, implying that separate N-terminal and C-terminal portions of Nad1 aremade in this system. Finally, in a search for primary transcripts, we isolated from a T. pyriformis mitochondrial fraction several small RNAs that were reproducibly labeled by incubation in the presence of [alpha-P-32]GTP and guanylyltransferase. Partial sequence information revealed that none of these cappable RNAs is encoded in the T. pyriformis mitochondrial genome. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 06:59:52