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Titolo:
New function for NF1 tumor suppressor
Autore:
Koivunen, J; Yla-Outinen, H; Korkiamaki, T; Karvonen, SL; Poyhonen, M; Laato, M; Karvonen, J; Peltonen, S; Peltonen, J;
Indirizzi:
Univ Oulu, Dept Anat & Cell Biol, FIN-90014 Oulu, Finland Univ Oulu OuluFinland FIN-90014 at & Cell Biol, FIN-90014 Oulu, Finland Family Federat Finland, Dept Med Genet, Helsinki, Finland Family Federat Finland Helsinki Finland pt Med Genet, Helsinki, Finland Univ Turku, Dept Surg, Turku, Finland Univ Turku Turku FinlandUniv Turku, Dept Surg, Turku, Finland Univ Turku, Dept Dermatol, Turku, Finland Univ Turku Turku FinlandUniv Turku, Dept Dermatol, Turku, Finland Univ Turku, Dept Med Biochem, Turku, Finland Univ Turku Turku FinlandUniv Turku, Dept Med Biochem, Turku, Finland
Titolo Testata:
JOURNAL OF INVESTIGATIVE DERMATOLOGY
fascicolo: 3, volume: 114, anno: 2000,
pagine: 473 - 479
SICI:
0022-202X(200003)114:3<473:NFFNTS>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROFIBROMATOSIS TYPE-1 GENE; INTERMEDIATE FILAMENTS; DROSOPHILA NF1; HUMAN SKIN; EXPRESSION; PRODUCT; KERATINOCYTES; PROTEIN; DIFFERENTIATION; MELANOCYTES;
Keywords:
cell adhesion; cytoskeleton; desmosome; neurofibromatosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Peltonen, J Univ Oulu, Dept Anat & Cell Biol, PB 5000, FIN-90014 Oulu, Finland Univ Oulu PB 5000 Oulu Finland FIN-90014 -90014 Oulu, Finland
Citazione:
J. Koivunen et al., "New function for NF1 tumor suppressor", J INVES DER, 114(3), 2000, pp. 473-479

Abstract

The expression and subcellular localization of neurofibromatosis type 1 tumor suppressor was studied in keratinocytes induced to differentiate by increased Ca2+ concentration of the culture medium. Differentiating keratinocytes became intensely immunoreactive for neurofibromatosis type 1 protein, which was apparently associated with cellular fibrils. Double immunolabelingwith antibodies to cytokeratin 14 and neurofibromatosis type 1 protein suggested an association of intermediate type cytoskeleton and neurofibromatosis type 1 protein. The presence of neurofibromatosis type 1 protein in cellpreparations treated with cytoskeletal buffer indicated a high affinity interaction between intermediate filaments and neurofibromatosis type 1 protein. Further studies utilizing double immunolabelings revealed that the intense neurofibromatosis type 1 tumor suppressor signal on intermediate filaments was temporally limited to the period in keratinocyte differentiation inwhich the formation of desmosomes takes place. Keratinocytes were also cultured from nine patients with type 1 neurofibromatosis and were studied with respect to cell morphology, and association of neurofibromatosis type 1 protein with intermediate cytoskeleton. The results showed that keratinocytes cultured from patients with neurofibromatosis type 1 displayed a highly variable cell size and morphology compared to controls. The latter findings represent predicted alterations in a situation where cytoskeletal organization is disturbed. Furthermore, differentiating neurofibromatosis type 1 keratinocytes were characterized by a reduced number of cytokeratin bundles that were decorated neurofibromatosis type 1 protein. The results of this study suggest that neurofibromatosis type 1 tumor suppressor exerts its effects in part by controlling organization of cytoskeleton during the formation of cellular contacts.

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Documento generato il 27/09/20 alle ore 13:37:10