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Titolo:
Synaptic localization of NMDA receptor subunits in the rat retina
Autore:
Fletcher, EL; Hack, I; Brandstatter, JH; Wassle, H;
Indirizzi:
Max Planck Inst Hirnforsch, D-60528 Frankfurt, Germany Max Planck Inst Hirnforsch Frankfurt Germany D-60528 Frankfurt, Germany
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 1, volume: 420, anno: 2000,
pagine: 98 - 112
SICI:
0021-9967(20000424)420:1<98:SLONRS>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROD BIPOLAR CELLS; NITRIC-OXIDE SYNTHASE; IONOTROPIC GLUTAMATE RECEPTORS; TUMOR-SUPPRESSOR PROTEIN; C-LIKE IMMUNOREACTIVITY; INNER PLEXIFORM LAYER; D-ASPARTATE RECEPTORS; AMINO-ACID RECEPTORS; GANGLION-CELLS; MAMMALIAN RETINA;
Keywords:
synaptic clustering; PSD-95; PSD-93; SAP 102; rod bipolar cells; NR1; NR2A; NR2B; NR1C2 ';
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
87
Recensione:
Indirizzi per estratti:
Indirizzo: Wassle, H Max Planck Inst Hirnforsch, Deutschordenstr 46, D-60528 Frankfurt, Germany Max Planck Inst Hirnforsch Deutschordenstr 46 Frankfurt GermanyD-60528
Citazione:
E.L. Fletcher et al., "Synaptic localization of NMDA receptor subunits in the rat retina", J COMP NEUR, 420(1), 2000, pp. 98-112

Abstract

The distribution and synaptic clustering of N-methyl-D-aspartate (NMDA) receptors were studied in the rat retina by using subunit specific antisera. A punctate immunofluorescence was observed in the inner plexiform layer (IPL) for all subunits tested, and electron microscopy confirmed that the immunoreactive puncta represent labeling of receptors clustered at postsynapticsites. Double labeling of sections revealed that NMDA receptor clusters within the IPL are composed of different subunit combinations: NR1/NR2A, NR1/NR2B, and in a small number of synapses NR1/NR2A/NR2B. The majority of NMDAreceptor clusters were colocalized with the postsynaptic density proteins PSD-95, PSD-93, and SAP 102. Double labeling of the NMDA receptor subunit specific antisera with protein kinase C (PKC), a marker of rod bipolar cells, revealed very little colocalization at the rod bipolar cell axon terminal. This suggests that NMDA receptors are important in mediating neurotransmission within the cone bipolar cell pathways of the IPL. The postsynaptic neurons are a subset of amacrine cells and most ganglion cells. Usually only one of the two postsynaptic processes at the bipolar cell ribbon synapses expressed NMDA receptors. In the outer plexiform layer (OPL), punctate immunofluoresence was observed for the NR1C2' subunit, which was shown by electron microscopy to be localized presynaptically within both rod and cone photoreceptor terminals. J. Comp. Neurol. 420:98-112, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:22:48