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Titolo:
Interaction between CD44 and the repeat domain of ankyrin promotes hyaluronic acid-mediated ovarian tumor cell migration
Autore:
Zhu, D; Bourguignon, LYW;
Indirizzi:
Univ Miami, Sch Med, Dept Cell Biol & Anat, Miami, FL 33101 USA Univ Miami Miami FL USA 33101 Dept Cell Biol & Anat, Miami, FL 33101 USA
Titolo Testata:
JOURNAL OF CELLULAR PHYSIOLOGY
fascicolo: 2, volume: 183, anno: 2000,
pagine: 182 - 195
SICI:
0021-9541(200005)183:2<182:IBCATR>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-ERYTHROCYTE ANKYRIN; INTERNAL CA2+ RELEASE; T-LYMPHOMA CELLS; TRANSMEMBRANE GLYCOPROTEIN GP85; BINDING DOMAIN; PLASMA-MEMBRANE; ANION-EXCHANGER; SIGNAL-TRANSDUCTION; ADHESION MOLECULE; CANCER CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Bourguignon, LYW Univ Miami, Sch Med, Dept Cell Biol & Anat, 1600 NW 10th Ave, Miami, FL 33101 USA Univ Miami 1600 NW 10th Ave Miami FL USA 33101 33101 USA
Citazione:
D. Zhu e L.Y.W. Bourguignon, "Interaction between CD44 and the repeat domain of ankyrin promotes hyaluronic acid-mediated ovarian tumor cell migration", J CELL PHYS, 183(2), 2000, pp. 182-195

Abstract

The adhesion molecule, CD44, interacts with ankyrin within its cytoplasmicdomain and binds to hyaluronic acid (HA) at its extracellular domain. In this study, we focused on the functional domain in ankyrin tin particular, the ankyrin repeat domain [ARD]) responsible for CD44 binding and its role in regulating HA-mediated ovarian tumor cell function. Using recombinant fragments of ankyrin (e.g., ARD and subdomain 1 [S1, aa1-aa217], subdomain 2 [S2, aa218-aa381], subdomain 3 [S3, aa382-aa612], and subdomain 4 [S4, aa613-aa834]) and in vitro binding assays, we determined that the S2 but not S1,S3, or S4 of ARD is the primary ankyrin binding region for CD44. Microinjection of antiglutathione S-transferase (GST)-tagged S2 or CST-tagged ARE fusion protein into CD44-positive ovarian tumor cells (e.g., SKOV3 cell line)promotes ankyrin association with CD44 in plaque-like structures and membrane projections. Additionally, we demonstrated that transfection of SKOV3 cells with S2cDNA or ARD cDNA results in an upregulation of HA-mediated tumor cell migration. Taken together, we believe that the S2 of the ARD plays apivotal role in the direct binding to CD44 and promotes the cytoskeleton activation required for HA-mediated function such as ovarian tumor cell migration, (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 09:09:06