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Titolo:
Direct extracellular contact between integrin alpha(3)beta(1) and TM4SF protein CD151
Autore:
Yauch, RL; Kazarov, AR; Desai, B; Lee, RT; Hemler, ME;
Indirizzi:
Dana Farber Canc Inst, Dept Canc Immunol & AIDS, Boston, MA 02115 USA DanaFarber Canc Inst Boston MA USA 02115 ol & AIDS, Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 h Med, Dept Pathol, Boston, MA 02115 USA Brigham & Womens Hosp, Div Cardiovasc, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 ardiovasc, Boston, MA 02115 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 13, volume: 275, anno: 2000,
pagine: 9230 - 9238
SICI:
0021-9258(20000331)275:13<9230:DECBIA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKEMIA-VIRUS TYPE-1; MHC CLASS-II; TRANSMEMBRANE-4 SUPERFAMILY; MONOCLONAL-ANTIBODIES; T-CELLS; ELECTRON-MICROSCOPY; SIGNAL-TRANSDUCTION; SYNCYTIUM FORMATION; C33 ANTIGEN; ASSOCIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Hemler, ME Dana Farber Canc Inst, Dept Canc Immunol & AIDS, Rm D-1430,44 Binney St, Boston, MA 02115 USA Dana Farber Canc Inst Rm D-1430,44 Binney StBoston MA USA 02115
Citazione:
R.L. Yauch et al., "Direct extracellular contact between integrin alpha(3)beta(1) and TM4SF protein CD151", J BIOL CHEM, 275(13), 2000, pp. 9230-9238

Abstract

Previously we established that the alpha(3)beta(1) integrin shows stable, specific, and stoichiometric association with the TM4SF (tetraspannin) protein CD151. Here we used a membrane impermeable cross-linking agent to show a direct association between extracellular domains of alpha(3)beta(1) and CD151. The alpha(3)beta(1)-CD151 association site was then mapped using chimeric alpha(6)/alpha(3) integrins and CD151/NAG2 TM4SF proteins. Complex formation required an extracellular alpha(3) Site (amino acids (aa) 570-705) not previously known to be involved in specific integrin contacts with otherproteins and a region (aa 186-217) within the large extracellular loop of CD151. Notably, the anti-CD151 monoclonal antibody TS151r binding epitope, previously implicated in alpha(3) integrin association, was mapped to the same region of CD151 (aa 186-217). Finally, we demonstrated that both NH2- and COOH-terminal domains of CD151 are located on the inside of the plasma membrane, thus confirming a long suspected model of TM4SF protein topology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 20:51:17