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Titolo:
Purification and partial characterization of creatine kinase from electricorgan of Electrophorus electricus (L.)
Autore:
Silva, CMBE; Tavares, NN; Giovanni-De-Simone, S; da Matta, AN; Hasson-Voloch, A;
Indirizzi:
Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisicoquim Biol, BR-21941 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21941 e Janeiro, Brazil Fundacao Oswaldo Cruz, Dept Bioquim & Biol Mol, Lab Microsequenciamento Prot, BR-21040900 Rio De Janeiro, Brazil Fundacao Oswaldo Cruz Rio De Janeiro Brazil BR-21040900 BCaneiro, Brazil Fundacao Tecn Educ Souza Marques, Escola Med, Dept Fisiol, Rio De Janeiro,Brazil Fundacao Tecn Educ Souza Marques Rio De Janeiro Brazil e Janeiro,Brazil
Titolo Testata:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
fascicolo: 4, volume: 32, anno: 2000,
pagine: 427 - 433
SICI:
1357-2725(200004)32:4<427:PAPCOC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROCYTE; CDNA;
Keywords:
creatine kinase; Electrophorus electricus (L.); purification; N-terminal amino acid sequence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Silva, CMBE Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisicoquim Biol, BR-21941 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21941 razil
Citazione:
C.M.B.E. Silva et al., "Purification and partial characterization of creatine kinase from electricorgan of Electrophorus electricus (L.)", INT J BIO C, 32(4), 2000, pp. 427-433

Abstract

The present investigation deals with the purification and the partial characterization of the soluble creatine kinase (CK) isoenzyme, isolated from the electric organ electrocyte of Electrophorus electricus (L.). Purification was performed by precipitation of the enzyme in the crude extract with ammonium sulfate (80%). The precipitate obtained was analyzed on an ion exchange column of diethylaminoethyl cellulose-52 (DEAE) followed by gel filtration on Superose 12 in a Fast Protein Liquid Chromatography (FPLC) system. Electrophoretic mobility of the active peak confirmed previous results identifying the hybrid isoenzyme MB in the electrocyte cytoplasm. Electrocyte CKis a dimeric enzyme with two identical subunits of approximately 40 kDa asestimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE). The: sequence analysis of the N-terminal peptide (14 amino acids) of the 40 kDa subunit showed homology with other CK enzymes from electric fish (Torpedo) and human muscle type CK. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:14:25